The results showed that Cd induced autophagy and DNA damage in GC-2 cells via ROS generation, and the autophagy signal pathway AMPK/mTOR was activated by ATM which is a DNA damage sensor. Melatonin, a well-known antioxidant, ameliorated DNA damage, and inhibited autophagy via the AMPK/mTOR signal pathway.

AMP触发的经典激活有三种具体机制：（1）促进LKB1 复合物对 Thr172 的磷酸化; (2) 抑制蛋白磷酸酶对Thr172 的去磷酸化; (3) 别构激活Thr172 磷酸化的 AMPK。 所有这三种效应都是由于 AMP 与 AMPK 的结合，而不是与上游激酶或磷酸酶的结合，这种三方机制确保系统以非常敏感的方式对 AMP 的小幅增加作出反应（ [1]: Trends Cell Biol. 2016）。 AMP通过三种效应激活AMPK。 Trends Cell Biol. 2016. ADP 的结合可以触发前两种机制，但大多数 AMPK 复合物对 AMP 的敏 …

2025年2月18日 · Here we show that in ATM glutaminolysis is the fundamental metabolic flux providing energy and substrate, bridging with AMP-activated protein kinase (AMPK) activity,...

2017年12月1日 · In our study, autophagy was activated by the AMPK/mTOR signaling pathway mediated by ATM following DNA damage induced by Cd, and autophagy in turn played a protective role in DNA damage.

2024年1月4日 · Purpose: AMP-activated protein kinase (AMPK) acts as a cellular energy sensor and is essential for controlling mitochondrial homeostasis. Here, we investigated the regulatory mechanisms involved in AMPK activation to elucidate how networks of intracellular signaling pathways respond to stress conditions.

2022年1月29日 · Here, to elucidate molecular mechanisms underlying increased expression and activity of ATM by AMPK under severe hypoxia, we investigated roles of transcriptional factors Sp1 and FoxO3a using human glioblastoma cell lines T98G and A172.

The ataxia-telangiectasia mutated (ATM) protein kinase is best known for its critical nuclear roles in the DNA damage response (DDR), cell cycle checkpoints, and the maintenance of gene stability. In this review, we highlight the multifaceted cytoplasmic functions of ATM in autophagy.

ATM regulates mTORC1 activity through AMPK in response to elevated ROS levels. Elevated ROS levels activate ATM, which in turn phosphorylates and activates LKB1 at Thr366, which then phosphorylates and activates AMPK at Thr172 . AMPK is also activated by IGF-1 and the AMPK activator AICAR in an ATM-dependent and LKB1-independent manner.

2023年1月1日 · ATM activation induced by DNA damage has been demonstrated to regulate cell autophagy via the AMPK/mTOR pathway (Zhang et al., 2015a), while mTOR is known to negatively regulate autophagy (Kou et al., 2016; Li et al., 2022b).

2008年4月23日 · ATM has been reported to phosphorylate the α subunit of AMP-activated protein kinase (AMPK), which senses AMP/ATP ratio in cells, and can be activated by upstream kinases. Here we provide evidence for a novel role of ATM in mitochondrial biogenesis through AMPK activation in response to etoposide-induced DNA damage.