ATP assays are procedures that can measure cell viability based on detection of ATP. Learn how ATP assays work and how to choose the right assays for your needs.

2013年5月1日 · The assay methods covered include the use of different classes of colorimetric tetrazolium reagents, resazurin reduction and protease substrates generating a fluorescent signal, the luminogenic ATP assay, and a novel real-time assay to monitor live cells for days in culture.

2022年7月6日 · ATP testing helps to detect the ATP produced by live microorganisms like bacteria from various surfaces and water, which helps assess the hygiene in the food industry and test the water quality quickly. But ATP testing does …

Detect and quantify ATP in biochemical and cell viability assays with the ATP Determination Kit. This luminescence-based ATP assay utilizes the luciferase-luciferin reaction, offering exceptional sensitivity to detect as little as 0.1 picomole of ATP using a luminometer or microplate reader.

In this article, we will specifically focus on the ATP bioluminescence assay, which uses the relationship between ATP, luciferin and luciferase to help determine the number of live cells. What is the ATP bioluminescence assay? How does the ATP bioluminescence assay work? Luciferase Reaction. What applications use the ATP bioluminescence assay?

2016年9月5日 · ATP检测试剂盒(ATP Assay Kit)可以用于检测普通溶液、细胞或组织内的ATP(adenosine 5'-triphosphate)水平。细胞和组织样品一步裂解即可完成样品制备，检测灵敏度高达1nM，化学发光可以持续稳定达30分钟，并且获得的样品还可以同时进行Western检测。

2017年7月6日 · Here, we describe detailed protocols to measure the ATP concentration in isolated living cells and animals using luminescence techniques based on targeted luciferase probes. In the presence of...

Cell viability assays are often used to screen collections of compounds to determine if the test molecules have effects on cell proliferation or show direct cytotoxic effects that eventually lead to cell death.

2023年9月4日 · ATP bioluminescence technology measures the presence of ATP in organic matter from various sources - living microbes, dead microbes, soil bioburden, and natural materials. The detection of ATP in bioburden is due to an enzymatic reaction resulting in bioluminescence.

The ATP cell viability assay utilizes luciferase to catalyze the formation of light from ATP and D-luciferin, which can be measured with a luminometer or Beta Counter.