2024年9月19日 · Here we report that tRNAs Ile from plant organelles and a subset of bacteria contain a new cytidine derivative, designated 2-aminovaleramididine (ava 2 C). Like L34, ava 2 C34 governs both...

2025年1月24日 · As a result, IF-3mt promotes the accurate initiation of leaderless mRNAs. Nevertheless, IF-3mt can also facilitate initiation from the non-AUG(AUA) start codon through its unique N- and C-terminal extensions, in concert with the 5-methylcytidine (m5C) or 5-formylcytidine (f5C) modification at the anticodon wobble position of mt-tRNAMet.

Identification of tRNAs present in H. marismortui, including the AUA-reading H. marismortui tRNA Ile XAU, was based on analysis of total RNA and not of individual purified tRNA species. We used (1) PAGE under denaturing conditions and acid urea PAGE followed by Northern blot analysis to identify specific tRNAs and their aminoacylation status ...

2003年9月1日 · The AUA codon-specific isoleucine tRNA (tRNA Ile) in eubacteria has the posttranscriptionally modified nucleoside lysidine (L) at the wobble position of the anticodon (position 34). This modification is a lysine-containing cytidine derivative that converts both the codon specificity of tRNA Ile from AUG to AUA and its amino acid specificity ...

In many bacteria, AUA codons are deciphered by tRNA Ile2 bearing lysidine (L) at the wobble position. L is a modified cytidine introduced post-transcriptionally by tRNA Ile -lysidine synthetase (TilS).

Deciphering AUA codons is a difficult task for organisms, because AUA and AUG specify isoleucine (Ile) and methionine (Met), separately. Each of the other purine-ending sense co-don sets (NNR) specifies a single amino acid in the universal genetic ...

Decoding of the AUA isoleucine codon in bacteria and archaea requires modification of a cytidine in the anticodon wobble position of the isoleucine tRNA. Here, we report the crystal structure of the archaeal tRNA 2Ile, which contains the novel modification agmatidine in its anticodon, in complex with the AUA codon on the 70S ribosome.

2011年10月16日 · Here we present the crystal structures of the Archaeoglobus fulgidus TiaS–tRNA Ile2 complexed with ATP, or with AMPCPP and agmatine, revealing a previously unknown kinase module required for...

Based on computational analysis, it has been proposed recently that a single intron-containing tRNA gene produces two very similar but functionally different tRNAs by means of alternative splicing; a UGG-decoding tRNA (TrpCCA) and an AUA-decoding tRNA (IleUAU).

2013年2月1日 · In many bacteria, AUA codons are deciphered by tRNA(Ile2) bearing lysidine (L) at the wobble position. L is a modified cytidine introduced post-transcriptionally by tRNA(Ile)-lysidine synthetase (TilS).