Among other factors consistent with the known pathways of entry and infection, the authors identified a new cellular factor that is required for infection of multiple AAV serotypes, which they term AAV receptor (AAVR).

2024年10月20日 · 摘要：腺相关病毒（AAV）是治疗遗传性疾病的新兴方法中的领先载体。 为了更广泛的临床应用，需要更高的转导效率和细胞特异性，这激发了对病毒-宿主分子在细胞进入过程中相 互作用的调查。 高通量方法更全面地鉴定宿主蛋白，随后的分子研究揭示了意想不到的复杂性和血清型特异性。 冷冻电子显微镜（cryo-EM）为这些有时是不均匀的病毒-宿主复合物的结构细节提供了一条途径，并且有望更充分地阐明进入的步骤。 这里介绍的，是理解糖基附加和受 …

2019年8月21日 · Adeno-associated virus (AAV) receptor (AAVR) is an essential receptor for the entry of multiple AAV serotypes with divergent rules; however, the mechanism remains...

2016年2月4日 · We show that AAVR directly binds to AAV2 particles, and that anti-AAVR antibodies efficiently block AAV2 infection. Moreover, genetic ablation of AAVR renders a wide range of mammalian cell types highly resistant to AAV2 infection. Notably, AAVR serves as a critical host factor for all tested AAV serotypes.

2016年1月27日 · We show that AAVR directly binds to AAV2 particles, and that anti-AAVR antibodies efficiently block AAV2 infection. Moreover, genetic ablation of AAVR renders a wide range of mammalian cell...

2019年2月11日 · The structure of adeno-associated virus (AAV) type 2 in complex with its receptor, AAVR, provides new insights on the molecular mechanism of AAV entry into host cells and will serve to optimize...

2023年3月20日 · 腺相关病毒 (Adeno-associated virus, AAV) 是一类无被膜、具有二十面体结构、直径20~26nm之间、含有4.70kb线性单链DNA基因组的细小病毒 [1]。AAV载体具有体内表达时间长、宿主范围广、免疫原性低、安全性高等优点, 是基因治疗研究中名副其实的“明星分子”。早在2012年, 第一个以AAV为基因载体的基因治疗药物Glybera在欧盟获批上市, 用于治疗脂蛋白脂肪酶缺乏症 (Lipoprotein lipase deficiency, LPLD) 。 随着AAV基础研究和临床试验的日益增多, 有 …

2023年9月20日 · Universal AAV receptor (AAVR) has been identified to be involved in cellular entry of different AAV serotypes. With the unveiling of the high-resolution AAV-AAVR complex structure by cryogenic electron microscopy, the atomic level interaction between AAV and AAVR has become the focus of study in recent years.

Adeno-associated virus (AAV) is a leading vector for virus-based gene therapy. The receptor for AAV (AAVR; also named KIAA0319L) was recently identified, and the precise characterization of AAV-AAVR recognition is in immediate demand. Taking advantage of a particle-filtering algorithm, we report her …

AAVR occurs at the cell surface and that AAVR serves as a cell surface receptor. Soluble AAVR (with PKD domains 1–5) bound directly to AAV2 particles in an enzyme-linked immunosorbent assay, and soluble AAVR as well as AAVR-specific antibody inhibited AAV2 infection in a concentration-dependent manner.