2015年8月1日 · Here, we have shown that AC5 and AC6 are the most abundant adenylyl cyclase isoforms in CGNPs, and that in both CGNPs and the chicken neural tube, these adenylyl cyclases exert a relevant effect on the Hh pathway. Additionally, AC5 and AC6 are among the few adenylyl cyclases isoforms that can be simultaneously regulated by Gα s and Gα i ...

2015年8月1日 · We found that overexpression of AC5 and AC6 represses, whereas their knockdown activates, the Hh pathway in CGNPs and in the embryonic neural tube. Indeed, AC5 and AC6 concentrate in the primary cilium, and mutation of a previously undescribed cilium-targeting motif in AC5 suppresses its ciliary location, as well as its capacity to inhibit Hh ...

2015年6月19日 · Here we show that AC5 and AC6 are the two isoforms most strongly expressed in cerebellar granular neuron precursors (CGNPs) and that that while over-expression of AC5 and AC6 represses the...

In this work, we show that AC5 and AC6 are the two adenylyl cyclase isoforms most strongly expressed in two well established Shh-target tissues: CGNPs and the embryonic neural tube. We also show that overexpression of AC5 and AC6 represses the Hh pathway, whereas their knockdown results in consistent activation

By using in ovo electroporation and fluorescent cell sorting, we studied the expression of endogenous AC1, AC3, AC5 and AC6 in the neuroblasts of HH- 12 chicken neural tube at 12 hours...

2006年3月27日 · Here, we will review recent discoveries regarding novel regulatory pathways for the calcium-inhibited adenylate cyclase isoforms (AC5 and AC6), focusing on posttranslational modifications. AC5 and AC6 are highly expressed in cardiac muscle and striatal neurons, however AC6 is also highly expressed in many other tissues (Defer et al., 2000).

We show that AC5/6 are key players in regulating ciliary cAMP. Given the recent finding that PIP 3 regulates AC5/6 , we used selective pharmacological and genetic overexpression strategies to reduce PIP 3 and effectively reduce ciliary cAMP levels.

2007年12月10日 · An important associated finding is that the functional deletion of AC6 and AC5 results in only a 70% reduction in LV cAMP-generating capacity, which indicates that (1) there remains enough AC5, even though not detectable by Western blotting, to provide a cAMP response, or (2) AC isoforms are present in cardiac myocytes other than AC5 and AC6 ...

Download scientific diagram | Adenylyl cyclases require ciliary localization to regulate the Hh pathway. (A) CGNP cultures were transfected for 24 h with HA-tagged constructs of AC1, AC3, AC5 or ...

2024年4月3日 · ac5 在心脏和血管功能中至关重要;ac6 是血管肌细胞中血管舒张途径的关键效应子，在胎儿/新生儿组织中富集。心力衰竭中 ac5 和 ac6 的表达均降低;然而，ac5 破坏具有心脏保护作用，而 ac6 的过表达在心脏损伤中挽救了心脏功能。