2024年12月5日 · N6 -Methyladenosine (m6A) is the predominant internal RNA modification in eukaryotic messenger RNAs (mRNAs) and plays a crucial role in mRNA stability. Here, using human cells, we reveal that m6A sites in the coding sequence (CDS) trigger CDS-m6A decay (CMD), a pathway that is distinct from previously reported …

In addition, an actinomycin D assay showed that the half-life of Runx2 mRNA was dramatically shortened in VSMCs overexpressing YTHDF2. These results suggest that YTHDF2 directly binds to the m6A modification site of Runx2 to mediate the mRNA degradation that prevents VC by inhibiting the osteogenic development of VSMCs.

2018年12月17日 · At 24 hpi, IFNB mRNA levels were measured by qRT-PCR (f), or cells were treated with actinomycin D and collected at 0, 0.5, 1 and 4 h post treatment (g).

One abundant mRNA modification is N6 -methyladenosine (m 6 A), which affects various aspects of RNA metabolism, including splicing, translation and degradation. Current knowledge about the proteins recruited to m 6 A to carry out these molecular processes is still limited.

2025年3月23日 · To this end, we systematically assessed the effects of m6A on mRNA half-life, translation efficiency, and alternative splicing across five cell lines (A549, HEK293T, HUVEC, JURKAT, and human embryonic stem cells (hESCs)) using actinomycin D-disrupted temporal transcriptome sequencing, ribosome sequencing, and ultra-high-depth transcriptome ...

2025年4月8日 · In our study, miR-146b-5p m 6 A modification was increased in osimertinib-resistant NSCLC cells by the m 6 A methyltransferase METTL16, and we found that m6A methylation mediated prolonged miR-146b-5p life cycle via Actinomycin D treatment and further miR-146b-5p quantitation.

The recovered protein was then identified by WB analysis. Actinomycin D experiment The RNA stability assay was conducted following established protocols [28]. Briefly, BMDMs were subjected to treatment with actinomycin D at a concentration of 5 µg/mL for specified durations (0, 20, 40, and 60 min) to assess RNA decay over time.

2025年2月10日 · An actinomycin D assay was conducted to analyze the effect of RBM15 silencing on XPR1 mRNA stability. A subcutaneous xenograft mouse model was established to validate the role of RBM15 and XPR1 in regulating the malignant behaviors of LUAD cells.

2025年3月1日 · METTL14 mediates m6A modification of TRPA1 mRNA to promote acute visceral pain caused by cervical dilatation. While TRPA1 serves as a therapeutic target for nociceptive pain, its role in acute visceral pain induced by uterine cervical dilation (UCD) remains an enigma.

We show that m6A regulates circZNF609 translation via YTHDF3 and. eIF4G2. N6-methyladenosine (m6A) is an RNA modification well-known for its contribution to different processes con-trolling RNA metabolism, including splicing, stability, and translation of mRNA.