The AEP2 gene has been isolated in this study from a wild-type yeast genomic library by genetic complementation of ts379 at the restrictive temperature. A 1740 nucleotide open-reading frame was observed that encodes a basic, hydrophilic protein of 67534 Da which possesses a putative mitochondrial address signal.

2008年12月12日 · The Aep2 protein binds to the 5′-untranslated region (UTR) of the OLI1 mRNA and is required for OLI1 mRNA translation. This interaction occurred in the hypothetical ancestral yeast species and was maintained in both Saccharomyces cerevisiae and Saccharomyces bayanus during evolution.

View all AEP2 alleles in SGD search. GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a …

2010年7月20日 · Using chromosome replacement lines of hybrids of two yeast species, a previous study identified a strong incompatibility between a S. bayanus nuclear gene, AEP2, and S. cerevisiae mitochondria that leads to interspecific F2 hybrid sterility .

Abstract The nucleotide sequence of the yeast nuclear AEP2 gene, required for the expression of the mitochondrial DNA- encoded subunit 9 of ATP synthase, predicts a primary transla- tion product of 67.5 kDa. The ATP13 gene is allelic to AEP2 but was reported to encode a protein of about 42 kDa in size. We thus

2008年12月12日 · We found that the hybrid line with Chromosome 13 substitution was completely sterile and identified Aep2, a mitochondrial protein encoded on Chromosome 13, to cause the sporulation defect as S. bayanus AEP2 is incompatible with S. cerevisiae mitochondria.

Present with 2680 molecules/cell in log phase SD medium. Showing features for mutagenesis. Suppresses a T-to-C nucleotide transition mutation in the 5' untranslated region of OLI1 mRNA localized 16 nucleotides upstream of the OLI1 reading frame. We now provide the "Disease & Variants" viewer in its own tab.

1995年7月24日 · The nucleotide sequence of the yeast nuclear AEP2 gene, required for the expression of the mitochondrial DNA-encoded subunit 9 of ATP synthase, predicts a primary translation product of 67.5 kDa. The ATP13 gene is allelic to AEP2 but was reported to encode a protein of about 42 kDa in size.

We found that the hybrid line with Chro-mosome 13 substitution was completely sterile and identified Aep2, a mitochondrial protein encoded on Chromosome 13, to cause the sporulation defect as S. bayanus AEP2 is incompatible with S. cerevi-siae …

AEP2. The inability to synthesize subunit 9 correlates with a lowered accumulation of the cognate olil mRNA indicating that the AEP2 product is involved in olil tran- script maturation or stabilization. The AEP2 gene has been isolated in this study from a wild-type yeast genomic