2019年5月10日 · Here, we identify and characterize the interactions of human MTR4 with a ribosome processing adaptor, NVL, and with ZCCHC8, an adaptor involved in the decay of small nuclear RNAs. We show that the unstructured regions of NVL and ZCCHC8 contain short linear motifs that bind the MTR4 arch domain in a mutually exclusive manner.

2022年6月9日 · Here, we determined cryogenic electron microscopy structures of human nuclear exosome targeting (NEXT) complexes bound to RNA that reveal mechanistic insights to substrate recognition and early steps that precede RNA handover to the exosome.

2019年7月29日 · Here, we identify and characterize the interactions of human MTR4 with a ribosome processing adaptor, NVL, and with ZCCHC8, an adaptor involved in the decay of small nuclear RNAs. We show that the...

Mtr4 is a Ski2-like RNA helicase that plays a central role in RNA surveillance and degradation pathways as an activator of the RNA exosome. Multiple crystallographic and cryo-EM studies over the past 10 years have revealed important insight into the Mtr4 structure and interactions with protein and nucleic acid binding partners.

2019年7月3日 · 6ro1: The MTR4 helicase recruits nuclear adaptors of the human RNA exosome using distinct arch-interacting motifs.

Page 2 uFll wwPDB X-ray Structure alidationV Report 6RO1 1 Overall quality at a glance O i The following experimental techniques were used to determine the structure: X-RAY DIFFRACTION The reported resolution of this entry is 3.07 Å. Percentile scores (ranging between 0-100) for global alvidation metrics of the entry are shown in the following ...

Molecular Components in 6RO1; Label Count Molecule; Proteins (2 molecules) A. 1: Exosome RNA Helicase Mtr4 (Gene symbol: MTREX) B. 1: Nuclear Valosin-containing Protein-like (Gene symbol: NVL) Chemicals and Non-standard biopolymers (8 molecules) 1. 6. SULFATE ION. 2. 1. ADENOSINE-5'-DIPHOSPHATE. 3. 1. CHLORIDE ION

6ro1 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. MTREX_HUMAN Component of exosome targeting complexes.

6RO1: X-ray crystal structure of the MTR4 NVL complex

6RO1 X-ray crystal structure of the MTR4 NVL complex. Summary for 6RO1. Entry DOI: 10.2210/pdb6ro1/pdb: Descriptor: Exosome RNA helicase MTR4, Nuclear valosin-containing protein-like, SULFATE ION, ... (6 entities in total) Functional Keywords: dexh helicase, complex, rna binding protein: