The Argonaute Proteins ALG-1 and ALG-2 Are Linked to Stress …
We characterized the loss-of-function mutants alg‑1 (gk214) and alg‑2 (ok304), which lack the two Argonaute-family members important for microRNA function, by using different proteotoxic stress assays (Grishok et al. 2001).
A mutation in Asparagine‐Linked Glycosylation 12 (ALG12) leads …
2020年6月2日 · Whole-genome resequencing revealed a mutation in asparagine-linked glycosylation 12 that encodes the mannosyltransferase responsible for adding the eighth mannose residue in an α-1,6 linkage to the dolichol-PP-oligosaccharide N …
Characteristics of ALG and EG in larval and adult brains. (A ...
A cytoplasmic reporter, RFP, and a nucleic reporter, Histone::YFP (hYFP), were used to label the somas and nuclei, respectively, when the membrane reporter was insufficient to identify the GAL4...
Mechanism of Ca2+-triggered ESCRT assembly and regulation of …
2014年12月23日 · These results demonstrate Ca 2+ -dependent accumulation of ESCRT III-Vps4 complex following large focal injury to the cell membrane and identify the role of ALG-2 as the initiator of sequential...
Annexin A7 is required for ESCRT III-mediated plasma membrane repair
2019年4月30日 · Here we provide evidence that ANXA7 upon plasma membrane injury is recruited to the site of injury where it forms a complex with the Ca 2+ -binding protein apoptosis-linked gene-2 (ALG-2). We...
2020年7月20日 · Alix (ALG-2 interacting protein X)/ PDCD6IP) is an adaptor 51 protein binding to ESCRT and endophilin-A proteins and thereby driving deformation and 52 fission of endosomal and cell surface membranes.
Larval EG generate adult ALG and EG. (A,B) Histone::YFP (hYFP ...
In addition to Type II NBs as a source of adult ALG, recent evidence demonstrates that some larval EG dedifferentiate to then generate adult ALG and EG during metamorphosis (Kato et al., 2020...
The Argonaute Proteins ALG-1 and ALG-2 Are Linked to
2021年10月14日 · During larval development in Caenorhabditis elegans, Argonaute-Like Gene 1 (ALG-1) is the primary mediator of the miRNA pathway, while the related ALG-2 protein is largely dispensable.
Using YFP as a reporter of gene expression in the green alga
Here, we describe the use of promoter-YFP fusions to profile promoter activity in response to metal availability in the green alga Chlamydomonas reinhardtii. YFP (Yellow Fluorescent Protein) is a general term used to refer to variants of GFP that carry amino acid substitutions that shift the protein to a yellowish emission (emission peak at 530 ...
黄色荧光蛋白 - 维基百科，自由的百科全书
黄色荧光蛋白（英語： Yellow Fluorescent Protein ，缩写YFP）是一种绿色荧光蛋白（GFP）的变体 [1] ，能发出黄色 荧光（激发峰517nm，发射峰527nm） [2] ，从GFP变为YFP至少需要蛋白质序列上四个氨基酸残基（aa）发生改变，其中最重要的是203号位的苏氨酸变为酪氨酸 ...