We fused the entire algD promoter, including the 5′ UTR, to gfp, essentially creating a transcriptional algD-gfp fusion. The algD-gfp construct was cloned into a mini-Tn 7 vector (pTn …

2007年11月15日 · We constructed mt-2(pPalgD-gfp) and mt-2(pPnptII-gfp), which harbor ectopic plasmid fusions of the promoters for algD and nptII, respectively. We examined the role of 1.5 …

2023年11月21日 · GFP-expressing Pst, Pst ΔalgU ΔmucAB (Pst algU mutant), and Pst ΔalgD ΔalgU ΔmucAB (Pst algU algD mutant) were examined to explore the roles of (1) alginate, a …

2003年6月16日 · The first gene of this operon, algD, encodes GDP mannose dehydrogenase, an enzyme essential for alginate synthesis . The algD gene product catalyzes the first committed …

2025年1月5日 · He et al. developed and evaluated novel amide derivatives as potent quorum-sensing inhibitors against P. aeruginosa PAO1 reporter strain, including lasB-gfp, rhlA-gfp, and …

本研究发现流行克隆似乎对囊性纤维化（CF）或非囊性纤维化（non-CF）个体有内在偏好，并发现了与CF亲和性正相关和负相关的基因表达特征。 高CF亲和性的克隆更能在CF巨噬细胞内 …

2018年9月25日 · We found that interactions between CdrA and the exopolysaccharide Psl fortify the matrix by preventing CdrA proteolysis. When both components—CdrA and Psl—are part of …

As shown in Fig. 2, the fluorescence of the arabinose-induced high-c-di-GMP-level strain (DGC1) was significantly higher than that of the uninduced DGC strain as well as the induced and …

During the course of chronic cystic fibrosis (CF) infections, Pseudomonas aeruginosa undergoes a conversion to a mucoid phenotype, which is characterized by overproduction of the …

2009年1月1日 · GDP-mannose dehydrogenase (GMD), encoded by the algD gene, is a key enzyme in the biosynthesis of alginate and catalyses the almost irreversible oxidation of GDP …