2022年9月5日 · To test whether ALK directly regulates the stability of PFKFB3 protein, cells were treated with cycloheximide (CHX), an inhibitor of translational elongation, with or without ceritinib...

2015年11月4日 · Cycloheximide (Naramycin A) 是一种抗真菌 (antifungal) 抗生素，是真核生物蛋白质合成 (protein synthesis) 的抑制剂，抑制体内蛋白质合成和 RNA 合成的 IC50 值分别为 532.5 nM 和 2880 nM。 Cycloheximide 可抑制铁死亡 (ferroptosis) 并抑制自噬 (autophagy)。 * Please select Quantity before adding items. • Cell. 2025 Feb 6;188 (3):814-831.e21. [Abstract] • Signal Transduct Target Ther. 2024 Sep 26;9 (1):254.

In this work, we have developed a highly controlled non-tumorigenic human bronchial epithelial cell system expressing four ALK fusions found in NSCLC. Employing RNA-seq and phosphoproteomics, we identify both common and isoform-specific differentially expressed genes and phosphorylated proteins downstream of these four ALK fusions.

2024年1月8日 · To test this hypothesis, we treated cells ectopically expressing wildtype, F1174S or F1174L mutant EML4-ALK V1, EML4-ALK V3a, and EML4-ALK V3b respectively with CHX, which blocks protein synthesis. Firstly, when expressed in NL20 cells, EML4-ALK V1 protein levels were lower than that of either EML4-ALK V3a or V3b ( Figure 5A ), in agreement ...

2023年2月15日 · In this work, we have developed a highly controlled non- tumorigenic human bronchial epithelial cell system expressing four ALK fusions found in NSCLC. Employing RNA-seq and phosphoproteomics, we identify both common and isoform- specific differentially expressed genes and phosphorylated proteins downstream of these four ALK fusions.

2024年4月8日 · Our findings revealed that the inhibition of anaplastic lymphoma kinase (ALK) and maternal embryonic leucine zipper kinase (MELK) selectively induces ERα degradation and prevents the...

2022年9月27日 · Our findings reveal a novel ALK-TOPK signaling pathway in ALK-positive NSCLC. The combination of alectinib and HI-032 might be a promising therapeutic strategy for improving the sensitivity of...

2011年1月13日 · NMP-ALK is a chimeric protein resulting from the (t2;5) (p23;q35) chromosomal translocation bringing the NPM gene at 5q35 in juxtaposition with the ALK gene at 2p23, which encodes a receptor tyrosine kinase expressed almost exclusively in the CNS during embryogenesis (Morris et al., 1997).

ALK基因融合是指ALK发生断裂并与其他基因融合，翻译后ALK融合蛋白构象改变，影响自身磷酸化，而导致肿瘤的发生。 ALK融合后的致瘤活性是由其融合伴侣决定的，ALK融合伴侣介导的ALK融合可不依赖其配体形成二聚体并使蛋白激酶域活化。 首个ALK融合蛋白 NPM1-ALK 于1994年在间变性大细胞淋巴瘤（anaplastic large-cell lymphoma，ALCL）细胞系中发现（这也是ALK蛋白被首次发现）。 此后，在多种恶性肿瘤中发现了多种类型的ALK融合蛋白。 临床前 …

CHX，中文名为放线菌酮，作用机理是通过结合到 80S核糖体 上进而阻止翻译过程 tRNA 的易位实现抑制蛋白质的合成。 一般来说，还有使用 MG132蛋白酶体抑制剂 来研究不同的降解机制。 知乎，中文互联网高质量的问答社区和创作者聚集的原创内容平台，于 2011 年 1 月正式上线，以「让人们更好的分享知识、经验和见解，找到自己的解答」为品牌使命。 知乎凭借认真、专业、友善的社区氛围、独特的产品机制以及结构化和易获得的优质内容，聚集了中文互联网科技、商业、 …