Bio-Rad offers a complete selection of the individual components contained in the Kallestad Autoimmune ANCA IFA Complete Kits. Components are available in a wide range of ordering options for maximum flexibility.

2024年8月11日 · Neutrophils contain 2 main types of granules: 1) peroxidase-positive (azurophilic), and 2) peroxidase-negative. Both ANCA-myeloperoxidase (MPO) and ANCA-proteinase 3 (PR3) are enzymes present in peroxidase-positive granules, and MPO is the most prevalent granular protein.

The combined application of ethanol- and formalin-fixed slides to detect atypical P-ANCA resulted in a lack of agreement between assays (κ, ≤0.39) in the interassay study and moderate agreement in the interobserver study (κ, 0.42). After atypical and typical P-ANCA patterns were combined, the consensus improved greatly.

2012年11月5日 · The ANCA consensus prescribes screening by indirect immunofluorescence on neutrophils. We evaluated the first automated ANCA-pattern recognition system. C-ANCA (n = 39) and P-ANCA (n = 40) samples were selected from …

ANCA Substrate Slides SLIDE: Slides containing human neutrophils stabilized and fixed directly on the test wells. Unique moat slide design minimizes cross contamination of wells during testing. The slide pouch is filled with an inert non-toxic gas that contributes to the stability of the cells.

2020年12月1日 · Detection of anti-neutrophil cytoplasmic antibodies (ANCA) by indirect immunofluorescence assays (IFA) is of diagnostic importance in vasculitides and some other inflammatory diseases. Automation of IFA may be beneficial in …

ANCA positive samples will demonstrate either nuclear (p-ANCA) or cytoplasmic (c-ANCA) staining dependent on antibody specificity. In formalin fixed slides, all ANCA antigens remain associated with the azurophilic granules causing both c-ANCA and

Formalin fixed slides for anti-neutrophil cytoplasmic antibody immunofluorescence assays (5 x 6 wells) 29418

Differentiation between C-ANCA, P-ANCA and possible cross reactivity with GS-ANA (Granular Specific ANA) should be made. C-ANCA is defined as an uneven granular staining of the cytoplasm. P-ANCA is defined as a perinuclear / nuclear staining, and is best differentiated on an ANCA IFA slide specifically prepared for P-ANCA detection (cat no: SA007).

2012年11月8日 · This document discusses antineutrophil cytoplasmic antibodies (ANCA). It notes that ANCA are autoantibodies related to inflammatory disorders and were first associated with Wegener's granulomatosis in 1985. The two main ANCA antigens are proteinase 3 and myeloperoxidase.