2016年5月9日 · Here we report the first non-fluorescent and membrane potential-sensitive SHG-active organic dye Ap3. Ap3 is photostable and generates SH signals at the plasma membrane with virtually no...

Here we report the first non-fluorescent and membrane potential-sensitive SHG-active organic dye Ap3. Ap3 is photostable and generates SH signals at the plasma membrane with virtually no...

Here we report the first non-fluorescent and membrane potential-sensitive SHG-active organic dye Ap3. Ap3 is photostable and generates SH signals at the plasma membrane with virtually no fluorescent signals, in sharp contrast to the previously used fluorescent dye FM4-64.

2020年3月23日 · Ap3 is photostable and generates SH signals at the plasma membrane with virtually no fluorescent signals, in sharp contrast to the previously used fluorescent dye FM4-64. When tested in neurons, Ap3-SHG shows linear membrane potential sensitivity and fast responses to action potentials, and also shows significantly reduced photodamage compared ...

Ap3是为了SHG成像而开发和合成的一种新型的无荧光性SHG专用染料，使用这种染料可实现观察其他荧光染料的同时观测。 利用这一点，使用Ap3进行SHG成像可以观察到响应神经活动引起的膜电位变化，同时通过钙敏感性染料的荧光成像，还可以观察到膜附近钙浓度的变化。 研究表明，使用Ap3进行的成像，由于光照射引起的信号衰减和细胞毒性非常低。 因此利用Ap3能够可视化细胞膜，捕捉膜电位的变化，还可能同时对荧光蛋白的动态和报告荧光染料的信号变化进行长时间 …

Ap3是为了SHG成像而开发和合成的一种新型的无荧光性SHG专用染料，使用这种染料可实现观察其他荧光染料的同时观测。 利用这一点，使用Ap3进行SHG成像可以观察到响应神经活动引起的膜电位变化，同时通过钙敏感性染料的荧光成像，还可以观察到膜附近钙浓度的变化。 研究表明，使用Ap3进行的成像，由于光照射引起的信号衰减和细胞毒性非常低。 因此利用Ap3能够可视化细胞膜，捕捉膜电位的变化，还可能同时对荧光蛋白的动态和报告荧光染料的信号变化进行长时间 …

2025年1月28日 · In this study, we investigated the interleaflet translocation characteristics of Ap3, an SHG-active dye molecule, using molecular dynamics (MD) simulations and two-dimensional random-walk (RW) simulations. The MD simulations revealed that Ap3 molecules only translocate between the leaflets along the pore sidewalls.

2016年5月9日 · Here we report the first non-fluorescent and membrane potential-sensitive SHG-active organic dye Ap3. Ap3 is photostable and generates SH signals at the plasma membrane with virtually no fluorescent signals, in sharp contrast to …

Ap3 is the world first Non-fluorescent, photostable and membrane potential-sensitive SHG-active organic dye. Second harmonic generation (SHG) imaging is a powerful tool to visualize cell / tissue structure and function, and widely used.

To overcome these fundamental limitations of fluorescent dye-based SHG imaging, we recently developed the Ap3 dye, specifically designed for SHG/SFG imaging that shows SHG/SFG signals without fluorescence (Nuriya et al. 2016). By using this SHG/SFG specific dye, it became possible, for the first time in the history of its application to biology ...