小贝: 从这两个对比图可以看出，这次实验APC-A750通道上的细胞群比正常实验的时候处于坐标轴低的位置。

Invitrogen Alexa Fluor 750 dye is a bright, near-infrared fluorescent dye with excitation well suited for the 633 nm laser line or dye-pumped laser excitation. For stable signal generation in imaging and flow cytometry, Alexa Fluor 750 dye is pH-insensitive over a wide molar range.

APC/Fire™ 750 was BioLegend’s first Fire tandem dye, originally developed to provide a more temperature and photostable alternative to APC/Cy7. APC/Fire™ 750 also has lower compensation requirements than APC/Cy7 conjugates while maintaining an equal level of …

The streptavidin, Alexa Fluor 750ûallophycocyanin (APC) streptavidin tandem conjugate has excitation and emission maxima at 650/779 and is widely used to detect biotinylated probes by flow cytometry in multicolor applications following e

After permeabilization with saponin-based permeabilization reagent IntraPrep, it may be used to detect intracytoplasmic CD3 by flow cytometry. CD3ζ see TCRζ in Human non-CD antigens section. The CD3 antigen is a complex of 5 invariable polypeptidic chains, γ, δ, ε, ζ and η, whose molecular weights are respectively 25-28, 21, 20, 16 and 22 kDa.

CD4 binds to a non-polymorphic region of MHC Class II molecules and is a co-receptor in MHC Class II restricted antigen-induced activation. CD4 is expressed on the T "helper" lymphocytes. It is present on most thymocytes where it is frequently co-expressed with CD8.

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After permeabilization with saponin-based permeabilization reagent IntraPrep, it may be used to detect intracytoplasmic CD3 by flow cytometry. CD3ζ see TCRζ in Human non-CD antigens section. The CD3 antigen is a complex of 5 invariable polypeptidic chains, γ, δ, ε, ζ and η, whose molecular weights are respectively 25-28, 21, 20, 16 and 22 kDa.

【产品名称】 通用名称:CD86-APC-A750检测试剂盒(流式细胞法) 英文名称:CD86 (B7-2)-APC-Alexa Fluor 750

【局限性】 由于荧光素的串联结构,APC-AlexaFluor750 也会在660 nm 处发光。 该二次发射峰因APC-AlexaFluor750的批间差异而不同。 因此对于多色分析,当APC-AlexaFluor750-结合物批次改变时,应仔细检查补偿矩阵。