2022年12月16日 · We describe how to characterize apoV size and morphology by nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM), and determination of specific biomarker expression by immunoblotting. Our protocol will be useful for preparing apoVs for further functional analysis.

The apothem (sometimes abbreviated as apo [1]) of a regular polygon is a line segment from the center to the midpoint of one of its sides. Equivalently, it is the line drawn from the center of the polygon that is perpendicular to one of its sides.

2024年9月5日 · Apoptotic vesicles (apoVs), small membrane vesicles generated during the apoptosis process, have gained attention for their role in regulating various physiological and pathological processes. In this study, we generated ciHep-derived apoVs (ciHep-apoVs) and investigated their therapeutic potential in alleviating liver fibrosis.

2024年8月6日 · Results: In this study, we show that loss of mechanical force led to cellular apoptotic resistance and aging related phenotype, thus reducing the number of apoVs in the circulation due to down-regulated expression of Piezo1 and reduced calcium influx.

2024年3月13日 · Two types of oxygen-related environmental stressed ApoVs are identified that directly target endothelial cells (ECs) for the accurate regulation of vascularization. Compared to normoxic and hypoxic ones, oxidatively stressed ApoVs (Oxi-ApoVs) showed the strongest tube formation capacity.

2022年9月1日 · Apoptotic vesicles (apoVs) are essential to maintain organ and tissue homeostasis. However, the characteristics and function of pluripotent stem cell (PSC)-derived apoVs (PSC-apoVs) are largely unknown. This study showed that PSC-apoVs produced 100 times more apoVs than human umbilical cord mesenchymal stem cells (UMSCs).

2024年9月20日 · Apoptotic vesicles (apoVs) play a vital role in various physiological and pathological conditions. However, we have yet to fully understand their precise biological effects in rescuing impaired mesenchymal stem cells (MSCs).

细胞凋亡 （Apoptosis）是程序性细胞死亡中一种主要方式，在组织和器官稳态维持中起到重要作用。 在凋亡过程中，细胞将发生皱缩、染色质凝集、细胞膜胀大膨出，最终产生一类包裹细胞内容物的双层脂质结构，即凋亡囊泡（Apoptotic vesicles, apoVs）。 作为近年来一类新兴的 细胞外囊泡 （Extracellular vesicles, EVs）群体­­，apoVs的发生过程、物理特性、生物学特征和体内生理病理状态下所发挥的生物学效应和分子机制尚未被人们所知。 进一步地，作为非细胞制剂，外 …

Mesenchymal stem cells (MSCs) influence T cells in health, disease and therapy through messengers of intercellular communication including extracellular vesicles (EVs). Apoptosis is a mode of cell...

Results: We show that systemic administration of mesenchymal stem cell (MSC)-derived apoptotic vesicles (MSC-apoVs) can ameliorate impaired ovarian folliculogenesis, PCOS phenotype, and reduced birth rate in Fasmut and FasLmut mice.