The atg17delta mutant forms a reduced number of small autophagosomes. As a result, it is defective in peroxisome degradation and is partially defective for autophagy. Atg17 interacts …

2012年12月21日 · ATG17-GFP cells display bright perivacuolar puncta, corresponding to the PAS in both SMD (SMD, synthetic minimal medium containing 0.67% yeast nitrogen base with …

Here we characterized Atg17/FIP200, the Drosophila ortholog of mammalian RB1CC1/FIP200, a proposed functional equivalent of yeast Atg17. Atg17 disruption inhibits basal, starvation …

2023年8月31日 · 通过体外GST pulldown、双荧光分子互补和体内免疫共沉淀等技术证实了Atg17和Atg23与Ypt1的互作是直接结合，随后鉴定了调控其结合的关键氨基酸残基。 破 …

ATG17 was first identified by a two-hybrid screen using ATG1 as bait (Kamada et al., 2000). ATG17 is required specifically for autophagy, but its precise role remains to be determined. …

An N-terminal GFP fusion to full-length Atg17 restored the Pho8Δ60 activity of the atg17Δ strain to ∼67% that of the wild-type strain, indicating that this construct retained substantial Atg17 …

2008年4月25日 · Approximately 30 autophagy-related (Atg) proteins have been identified in S. cerevisiae, 17 of which are essential for formation of the autophagosome (reviewed in 17). Null …

2016年1月1日 · The Atg17-Atg31-Atg29 Complex Interacts with the Vam7 SNARE Protein at the PAS. The Atg17-Atg31-Atg29 complex is a scaffold that is vital for PAS organization during …

2012年12月21日 · Atg17 is crescent shaped with a 10 nm radius of curvature. Dimerization of the Atg17-Atg31-Atg29 complex is critical for both PAS formation and autophagy, and each dimer …

Two-hybrid analyses and coimmunoprecipitation experiments demonstrated that Atg17 physically associates with Atg1-Atg13 complex, and this binding was enhanced under starvation …