2017年9月13日 · As the Atg4 activity is essential and highly specific to autophagy, Atg4 is a prospective target for developing autophagy-specific inhibitors. In this review article, we …

LC3/Atg8 的 C 端被 Atg4 蛋白酶酶切后生成细胞质 LC3-I。LC3-I 与磷脂酰乙醇胺 （PE） 也以泛素样反应的方式连接，这个反应需要 Atg7 和 Atg3 （分别为 E1 和 E2 样酶）。LC3 的脂质形 …

自噬（autophagy=self-eating）意为自体吞噬，是真核细胞在自噬相关基因（autophagy related gene，Atg）的调控下利用溶酶体降解自身细胞质蛋白和受损细胞器的过程。 自噬可防止细胞 …

Atg5-Atg12-Atg16L复合物 具有类E3连接酶活性, 主要通过激活Atg3酶活性, 促进LC3（也就是Atg8）从Atg3转移到底物磷脂酰乙醇胺(PE)上。一旦自噬体形成后，Atg5-Atg12-Atg16L复合 …

LC3-I 也是通过一个需要 Atg7 和 Atg3（分别对应 E1 和 E2 样酶）参与的泛素样反应，结合到磷脂酰乙醇胺 (PE)。 脂质化形式的 LC3，也称作 LC3-II，附着到自噬体的膜上。

Atg4 cleaves pro-Atg8 to form Atg8-I, exposing a key glycine residue at the C terminus. This step is referred to as “priming” and allows for the conjugation of Atg8 to phosphatidylethanolamine …

2016年4月1日 · Atg4 proteases are cysteine proteases, which are well known for their crucial roles in the lipidation and delipidation of LC3 during the autophagy process. At least four …

Atg8 is constitutively cleaved by Atg4 and upon autophagosome biogenesis induction, it is conjugated to the phosphatidylethanolamine (PE) present in autophagosomal membranes. …

2017年8月18日 · It has been shown that Atg4 constitutively deconjugates Atg8 from PE on all membranes except the PAS, suggesting the existence of regulatory elements protecting …

As the Atg4 activity is essential and highly specific to autophagy, Atg4 is a prospective target for developing autophagy-specific inhibitors. In this review article, we summarize our current …