Atg8-PE protein-based in vitro biochemical approaches to …
In this review, we comprehensively summarize Atg8-PE protein-based in vitro biochemical approaches and recent advances to facilitate a better understanding of autophagy …
你不可不知的PTM最新机制！会是下一个国自然热点吗？ - 知乎
在两个类泛素化系统的作用下，细胞质可溶的atg8蛋白连上脂质分子pe，成为膜结合形式的atg8-pe分子，进而定位于自噬体膜。 由于ATG8-PE具有稳定的自噬体定位特征，ATG8-PE一直是 …
Atg8, a Ubiquitin-like Protein Required for ... - ScienceDirect
2007年7月13日 · Our results suggest that the membrane-tethering and hemifusion functions of Atg8 are evoked by the conjugation with PE, whereas Atg4 functions as a deconjugase that …
上海交大薛红卫教授课题组揭示相分离参与调控细胞自噬的分子机 …
2021年10月25日 · 自噬的核心是泛素折叠蛋白ATG8，经脂质磷脂酰乙醇胺（PE）修饰后结合吞噬体和自噬体。 ATG8一旦插入自噬膜，脂质化的ATG8为众多自噬适配体和受体提供了一个对 …
Membrane perturbation by lipidated Atg8 underlies ... - Nature
2021年7月8日 · To obtain structural insights into the activity of Atg8–PE to induce membrane area expansion and deformation, Atg8–PE was reconstituted onto lipid bilayer nanodiscs by in vitro …
ATG8 lipidation and ATG8‐mediated autophagy in Arabidopsis …
2010年4月16日 · In this study, we developed a method to reliably detect the ATG8-PE adduct and demonstrate its absence in backgrounds containing previously reported or new mutant alleles …
Activation and targeting of ATG8 protein lipidation
2020年5月5日 · ATG8 lipidation is a hallmark of autophagy where these proteins are involved in autophagosome formation, the fusion of autophagosomes with lysosomes and cargo selection. …
Crystal Structure of Autophagy-Associated Protein 8 at 1.36 Å ...
2025年3月11日 · The ATG8-PE conjugate is predominantly located on both sides of the autophagic vesicle membrane, and ATG4 removes ATG8-PE from the outer membrane as the …
基于 Atg8–PE 蛋白的自噬研究体外生化方法,Autophagy - X-MOL
最近，已经成功开发了几种生化方法来在体外生产脂化蛋白 Atg8-PE 或其模拟物，包括酶介导的重构系统、化学成分确定的重构系统、无细胞脂化系统和蛋白质化学合成。
Complete set of the Atg8–E1–E2–E3 conjugation machinery
2023年12月6日 · Here we show that Saccharomyces cerevisiae Atg8–PE and E1–E2–E3 enzymes together construct a stable, mobile membrane scaffold. The complete scaffold …