2016年3月22日 · Here we identify a Myc-like HLH transcription factor network comprised of Mondo/Max-like complex (MML-1/MXL-2) to be required for longevity induced by germline removal, as well as by reduced...

2024年9月3日 · In this work, we describe how metabolic inputs regulate MML-1 subcellular localization and function. We found that HXK-1 and HXK-2 regulate MML-1 cellular distribution and are required for germline longevity through the regulation of mitochondrial β-oxidation and the pentose phosphate pathway (PPP), respectively.

基于神经元 rna 干扰 (rnai) 的转录组分析表明，谷氨酸转运蛋白 glt-5 是 mml-1 的下游靶标，但不是 hlh-30。 此外，在长寿动物中，神经元中的 MML-1-GTL-5 轴对于防止年龄依赖性蛋白质稳态崩溃以及分别通过自噬和过氧化物酶 MLT-7 增加氧化应激至关重要。

2023年9月9日 · Our study provides evidence that intertissue-communications by neuronal MML-1/MXL-2 result in systemic regulation that is crucial for controlling organismal aging. Accumulating evidence has demonstrated the presence of intertissue-communication regulating systemic aging, but the underlying molecular network has not been fully explored.

MML-1 is a 1009 amino acid protein with multiple conserved domains, including the N-terminal Mondo Conserved Regions (I-V) (Pickett et al. 2007), which form a glucose sensing module in ChREBP (Abdul-Wahed et al. 2017), a proline-rich region, similar to that in ChREBP (Ortega-Prieto and Postic 2019), and a C-terminal DNA-binding and MXL-2 heterod...

2024年5月5日 · These findings support our conclusion that neuronal MML-1/MXL-2 preferentially plays a role in glp-1 longevity. In this way, we utilized original transgenic lines other than TU3401, conducted complementary genetic experiments, and performed extensive lifespan analyses to corroborate our findings.

MML-1/MXL-2 in the regulation of lifespan in Caenorhabditis elegans. We hope our response adequately addressed the concerns and reinforced our findings’ validity. 1. S. Gahlot, J. Singh, C. elegans neuronal RNAi does not render other tissues refractory to RNAi. 2. A. Calixto et al., Enhanced neuronal RNAi in C. elegans using SID-1. Nat.

目的 分析Fas诱导的B淋巴瘤细胞株MML-1凋亡过程中，PMA阻断MML-1进入细胞周期后细胞凋亡敏感性降低的原因。方法 采用10 nmol/L PMA预处理MML-1细胞24 h，用流式细胞仪检测PMA阻断细胞周期前后50 ng/mL抗Fas抗体诱导的MML-1细胞凋亡率的变化。采用PI-Triton X …

采用PI-Triton X和FITC-active caspase-3双染色分析经Fas诱导凋亡的MML-1内活化caspase-3的表达。以细胞周期蛋白cyclin A、B 1 、E标记Fas诱导凋亡的处于不同增殖周期MML-1（S、G 2 /M和G 1 期细胞），流式细胞术检测细胞内活化caspase-3的表达。结果 Fas

B-1 B cells are chiefly responsible for maintaining preimmune humoral defense at homeostasis. 1 Their biased immunoglobulin (Ig) repertoire and capacity for generating broadly reactive antibodies are not only tailored for basal protection and primary immune reactivity, but are also fundamental for housekeeping functions, such as removing ...