2023年8月28日 · Genomic instability can induce an anti-tumour immune response via activation of the cGAS-STING pathway following the formation of micronuclei (MN). Here, the authors identify a role for DNAJA2 in...

2019年8月13日 · We treated OVA-expressing B16 mouse melanoma cells (B16-OVA) with an FDA-approved drug library for 16 hours, cocultured with bone marrow–derived DCs (BMDCs) and OVA-specific CD8 + T cell hybridoma B3Z cells for 24 hours, then measured IL-2 promoter–driven LacZ activity, which reflected IL-2 expression .

2024年12月31日 · The nuclear envelope encases the genetic material, and NE rupture results in DNA leakage to cytosol. Thus, we engineered the B16 H2B-RFP cells to express the fusion protein cGAS-GFP (B16 cGAS-GFP/H2B-RFP, also used Hepa1-6 cGAS-GFP cell) and inoculated these cells to VHPV metastasis model.

2017年2月14日 · Here we show that cGAS is indispensable for the antitumor effect of immune checkpoint blockade in mice. Wild-type, but not cGAS-deficient, mice exhibited slower growth of B16 melanomas in response to a PD-L1 antibody treatment.

Inspired by the melanoma tumor results, we assessed PRMT5 function in antitumor immunity in B16F10 (B16) metastatic murine melanoma cells expressing either scrambled (Scr) or PRMT5-specific short hairpin RNA (shRNA). PRMT5 knockdown (KD) in B16 cells reduced PRMT5 abundance and decreased PRMT5 activity .

Detection of cytosolic DNA by the enzyme cGAS triggers the production of cGAMP, a second messenger that binds and activates the adaptor protein STING, which leads to interferon (IFN) production. Here, we found that in vivo natural killer (NK) cell killing of tumor cells, but not of normal cells, depends on STING expression in non-tumor cells.

2024年5月16日 · Further in vivo and in vitro analyses reveal that zebularine stimulates cGAS-STING-NF-κB/IFNβ signaling to enhance tumor cell immunogenicity and upregulate antigen processing and...

2019年7月22日 · Here, we show that the receptor tyrosine kinase HER2 (also known as ErbB-2 or Neu) potently inhibits cGAS–STING signalling and prevents cancer cells from producing cytokines, entering senescence...

2023年10月9日 · cGAS-STING pathway is a natural immune signaling pathway, the activation of which can secrete the type I interferons (IFNs) to promote dendritic cell (DC) maturation, and subsequently increased immune cell infiltration into the tumor microenvironment (TME) (e.g., natural killer cells [NKs] and cytotoxic T cells [CTLs]), thus eliciting an anti ...

2021年10月21日 · Our study reports phase separation-mediated quiescence of cGAS-STING signaling by a mutant tumor suppressor and reveals gain-of-function pathogenesis for NF2-related tumors by regulating antitumor immunity.