Using Microsoft Excel to plot and apply standard curve A protein assay, such the BCA Protein Assay, is an excellent tool for estimating the protein concentration of a sample. The intensity of the colored reaction product is a direct function of protein amount that can be determined by comparing its absorbance value to a standard curve.

The Thermo Scientific™ ™Pierce BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in

Example standard curves for the Thermo Scientific Pierce BCA Protein Assay Kit. Eight concentrations of bovine serum albumin (BSA) and bovine gamma globulin (BGG) were assayed. The response values (absorbances) were plotted and a best-fit line drawn through the points.

The bicinchoninic acid (BCA) assay is one of many ways to measure the total protein concentration of an unknown sample. In this assay, copper (II) [Cu 2+] is reduced to copper (I) [Cu 1+] in the presence of peptides in alkaline solutions. BCA can chelate (bind to) Cu 1+, creating a purple color

Pierce Dilution-Free Rapid Gold BCA Protein Assay generates a linear standard curve across a wide dynamic range (0–10 mg/mL). Standard curves were produced for the Pierce Dilution-Free Rapid Gold BCA Protein Assay and the Quick Start Bradford Protein Assay (Bio-Rad) with purified BSA in 0.9% saline (0–10 mg/mL).

The Pierce BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein.

In simple terms you need to see the equation Y=mX+C, which can be generated by plotting the std conc in Xaxis and absorbance in Y. You will get a linear line and excel can generate the equation...

2018年1月26日 · Utilizing Beer’s law, known concentrations of bovine serum albumin (BSA) are reacted with bicinchonic acid (BCA) to create a standard curve of absorbance vs. concentration of protein. This curve is then utilized to determine the concentration of protein in …

Principle of Bicinchoninic Acid (BCA) Protein Assay. The Bicinchoninic Acid (BCA) protein assay is a widely used method for the quantification of total protein in a sample. The BCA assay relies on the reduction of Cu 2+ ions to Cu 1+ ions by protein in an alkaline medium.

The bicinchoninic acid (BCA) assay, first described by Smith et al. (1) is similar to the Lowry assay, since it also depends on the conversion of Cu 2+ to Cu + under alkaline conditions (see Chapter 2). The Cu + is then detected by reaction with BCA.