2013年8月6日 · H89 is a protein kinase A (PKA) inhibitor that blocks COPII-coated vesicle assembly by preventing SAR1 binding to ER membrane when used at a high concentration (Chijiwa et al., 1990; Aridor and Balch, 2000). Brefeldin A (BFA) is a fungal metabolite that inhibits ARF1 activation (Peyroche et al., 1999).

H89 Inhibits Hypotonically induced-, BFA-induced-, and Nocodazole-induced Stimulation of Golgi-to-ER Transport. In a search for pharmacological agents that prevented the hypotonically induced stimulation of Golgi-to-ER retrograde transport, we tested a panel of drugs known to inhibit a variety of signaling molecules.

2003年9月15日 · Brefeldin A (BFA), an inhibitor of intracellular protein translocation, blocked the stimulatory action of H89 on the Na + absorption by interrupting the action of H89 on the Po and number of the NSC channel. H85, an inactive form of H89, showed an effect similar to H89, suggesting that H89 does not show its effect by inhibiting PKA, but acts on ...

In one, ER redistribution was achieved using a combination of brefeldin A (BFA) to cause Golgi collapse and H89 to block ER export. Unlike brefeldin A alone, which leaves matrix proteins in relatively large remnant structures outside the ER, the addition of H89 to BFA-treated cells caused ER accumulation of all Golgi markers tested.

BFA/H89 treatment produces ER localization of Golgi membrane proteins with a more granular redistribution of GM130. Wild-type HeLa cells were treated with BFA supplemented with H89, and then cells were fixed and costained for Sec61p (B) with GalT (A), Giantin (C), or …

BFA/H89 disperses GalNAcT2-GFP and GM130 into ER-like structures with extensive ER exit site breakdown. HeLa cells stably expressing GalNAcT2-GFP were left untreated (A, B, and G),...

Brefeldin A (BFA) 处理 15 小时或 40 小时，会导致内质网 (ER) 显著肿胀，并将其定位转移到正常大鼠肾 (NRK) 细胞的外围。 延长 Brefeldin A 处理会导致 MT 和肌动蛋白细胞骨架显著破坏 [1] 。

Having confirmed that BFA/H89 treatment produces extensive redistribution of Golgi proteins to the ER, we asked if BFA/H89 washout leads to rapid resumption of secretory trafficking in HeLa...

2021年4月13日 · We used H89, a kinase inhibitor known to inhibit Sar1 recruitment to the ER (Aridor and Balch, 2000; Lee and Linstedt, 2000), to disrupt COPII assembly. Adding H89 to cells caused a rapid and sustained decrease in the intensity of Sec23 puncta, especially at the cell periphery, without noticeable changes in the ER and Golgi structure over the ...

2003年11月11日 · Recently, Golgi disassembly caused by Arf1 inactivation in BFA-treated cells was shown to be blocked by pretreatment of cells with the small molecule H89, an inhibitor of PKA, and other kinases . This finding prompted us in this study to investigate whether H89 could also inhibit mitotic Golgi disassembly and, if it could, to determine whether ...