Recruitment of LC3 to damaged Golgi apparatus | Cell Death ...
2018年10月22日 · LC3 is a protein that can associate with autophagosomes, autolysosomes, and phagosomes. Here, we show that LC3 can also redistribute toward the damaged Golgi apparatus where it clusters with...
Autophagy induction via STING trafficking is a primordial function …
2019年3月6日 · To study DNA-induced autophagy, we transfected interferon-stimulatory DNA (ISD) into BJ cells, which are immortalized human fibroblasts. DNA transfection not only activated downstream components of...
布雷菲德菌素A对HepG2细胞自噬的诱导作用 - 百度学术
为探讨新型抗癌药物布雷菲德菌素A(BFA)诱导肝癌细胞发生自噬性死亡的作用机制,以HepG2细胞作为研究对象,将细胞分成阴性对照组,不同浓度BFA组和阳性对照组(雷帕霉素),采用MTT法测定HepG2细胞活性;采用免疫荧光法检测细胞内自噬标志蛋白LC3的表达和细胞核形态学 ...
Brefeldin A delivery nanomicelles in hepatocellular carcinoma …
2021年10月1日 · In this study, we designed a novel polymeric nanomicelle based on the pluronic and polyethylene glycol 1000 vitamin E succinate (TPGS) to deliver BFA. TPGS is a water-soluble derivative of natural vitamin E which has been used as an absorption enhancer, emulsifier, and permeation enhancer in DDS [37], [38].
布雷菲德菌素A对HepG2细胞自噬的诱导作用 - RCEES
结果表明，BFA可引起HepG2细胞内自噬特征蛋白LC3-Ⅱ、Atg5、Beclin-1的蛋白表达水平明显增加；同时引起HepG2细胞活性降低、细胞数量减少、细胞核固缩和变形。
Brefeldin A induced inhibition of de novo globo- and neolacto …
1992年12月15日 · In order to account for BFA-induced accumulation of Lc3, the effect BFA of a as direct inhibitor of pl+ 4galactosyltransferase activity was tested using a crude cell homogenate of HCT-15 cells. These results are shown in 1.
Golgi-associated LC3 lipidation requires V-ATPase in ... - Nature
2016年8月11日 · AMDE-1-induced LC3 lipidation was colocalized with the Golgi complex, and was inhibited by the disturbance of Golgi complex. The integrity of the Golgi complex was also required for multiple...
Regulation of autophagic flux by MSNs-BFA. (a) LC3
The LC3 level of cells treated with MSNs-BFA is even higher than that of cells treated with BFA alone, which is attributed to accumulation of autolysosomes by BFA induction and the...
NF-κB通路与内质网应激诱导的宫颈癌细胞自噬和细胞凋亡相关 …
2017年1月1日 · BFA增加了TC-1和HeLa细胞中LC3的点状染色以及通过TEM观察到的自噬体的数量。还评估了自噬通量。阻滞溶酶体中LC3降解的Bafilomycin导致LC3I和LC3II积累。BFA通过激活caspase-12 / caspase-3途径启动TC-1肿瘤细胞的凋亡。
Brefeldin A inhibits colorectal cancer growth by triggering …
2019年1月22日 · We found that BFA treatment increased the interaction of Beclin 1 with ATG14L and decreased the binding of Beclin 1 with Bcl-2 (Fig. 2C). Moreover, either treatment with 3-MA (an inhibitor of class III PI3K) or siRNA-mediated ATG5 silencing prominently restored elevation of LC3-II levels in BFA-treated cells (Fig. 2D and Supplemental Fig. S1F ...