2022年10月29日 · In this study, BRD4-binding proteins were obtained through co-immunoprecipitation (Co-IP) and mass spectrum (MS) to identify the co-regulators with BRD4 during transcription enhancement (Fig....

2023年9月22日 · To determine which PRMTs methylated BRD4, we assessed the potential interactions between PRMTs (PRMT1, PRMT2, PRMT3, PRMT4, PRMT5, and PRMT9) and BRD4 by using immunoprecipitation (IP) and ...

2017年12月20日 · Using two conditional knockout mouse strains and derived cells, we demonstrate that Brd4 controls cell identity gene induction and is essential for adipogenesis and myogenesis. Brd4...

2019年11月11日 · The bromodomain protein 4 (BRD4) is an atypical kinase and histone acetyl transferase (HAT) that binds to acetylated histones and contributes to chromatin remodeling and early transcriptional elongation. During transcription, BRD4 travels with the elongation complex.

2023年8月17日 · Although BRD4-PTEFb can associate with chromatin through acetyl recognition, our results indicate that a distinct, active BRD4-PTEFb population functions to regulate transcription independently of bromodomain-mediated chromatin association. These findings may enable more effective pharmaceutical modulation of BRD4-PTEFb activity.

2025年1月3日 · We found that targeted inhibition of bromodomain-containing protein 4 (BRD4) by siRNA or its inhibitor (JQ1) significantly decreases mRNA m 6 A levels and suppresses the malignancy of breast cancer (BC) cells via increased expression of …

brd4 是一种转录和表观遗传调节因子，在胚胎发生和癌症发展过程中起着关键作用。作为溴结构域和末端外结构域 (bet) 家族的成员，brd4 的特征在于具有两个串联溴结构域（bd1、bd2）。bds 结合靶蛋白（包括组蛋白）…

2025年2月27日 · 近日，一项由宁波大学医学院附属妇女儿童医院与同济大学附属东方医院妇产科合作的研究，揭示了BRD4（Bromodomain-containing protein 4）在PCOS中的重要作用，以及其与内质网应激（ERS）的复杂关系。

近日，尤涵教授研究团队在PNAS上发表题为A deregulated m6A writer complex axis driven by BRD4 confers an epitranscriptomic vulnerability in combined DNA repair–targeted therapy的研究论文，该工作揭示了BRD4通过调控MTC影响m6A 丰度 及其在BETi/PARPi 联合靶向治疗 中的功能。

2013年3月7日 · Using purified proteins for individual incubation with recombinant FLAG-tagged human Brd4 (f:Brd4) and followed by immunoprecipitation (IP) with anti-Brd4 antibody and immunoblotting (IB) using antibodies recognizing tested proteins, we found that none of the human RNA polymerase II (Pol II) general transcription components (TFIIA, TFIIB, TFIID ...