2018年12月1日 · Herein, the primary structure of BSA was analyzed by SDS-PAGE, secondary structure by Circular Dichroism (CD), and tertiary structure by Fluorescence and SAXS spectroscopy. The zeta-potential measurements shed light …

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.

2023年10月18日 · Biological solution SAXS (BioSAXS) is routinely employed at synchrotrons to study biological molecules in solution to determine the scattering form factors and intermolecular...

2023年3月24日 · Here, we present a novel analytical method using small-angle X-ray scattering (SAXS) to solve the structure of bovine serum albumin (BSA) while encapsulated within two zeolitic imidazolate frameworks (ZIF-67 and ZIF-8).

2006年12月10日 · We have studied a series of samples of bovine serum albumin (BSA) solutions with protein concentration, c, ranging from 2 to 500 mg/mL and ionic strength, I, from 0 to 2 M by small-angle X-ray scattering (SAXS).

2003年6月15日 · Classical parameters obtained from surface tension technique coupled to small angle X-ray scattering (SAXS) measurements gave support to investigate conformational changes in the bovine serum albumin (BSA)-sodium dodecyl sulfate (SDS) complexes, as well as the size of the micelle-like clusters distr …

2019年8月30日 · We newly developed Laboratory-based Size exclusion chromatography SAXS System (La-SSS) by utilizing a latest laboratory-based SAXS instrument and finely optimization of the balance between...

小角X射线散射 （Small Angle X-ray Scattering, SAXS）是指当X射线照射到试样上，在靠近入射X光束周围2°~5°的小角度范围内发生的散射现象。 由于电磁波的散射遵循 反比定律 ——被照物体的有效尺寸相对于入射波越大，电磁波的散射角越小，因此SAXS的研究主体主要是数百至上千埃的亚微观结构物质（高聚物、蛋白质、溶胶凝胶、超细粉末、多孔碳等）。 总之，SAXS技术凭借其得天独厚的优势，已成为亚微观结构研究的主要工具之一。 图1 X射线与物质的相互作用. …

2016年7月1日 · Protein folded/unfolded states can be detected by SAXS and fluorescence experiments. The pH and the ionic strength affect the apparent molecular weight of the BSA. The BSA in E-form can bind more hydrophobic molecules than in N-form. FL and ITC methods provides correct thermodynamic data for the BSA/ligand interactions.

2018年12月1日 · CD analyzed that at premicellar concentration, metallosurfactants stabilize the helical structure of BSA and at post-micellar concentration, α-helicity decreases nearly to 80%. Similar observations were made by SAXS study that indicates the unfolding of BSA at post micellar concentration.