2025年2月11日 · To gain molecular insights into the steps preceding toxin translocation, we determine cryo-EM structures of BoNT/A1 alone and in complex with its receptor synaptic vesicle glycoprotein 2B (SV2B)....

2006年4月28日 · How the widely used botulinum neurotoxin A (BoNT/A) recognizes and enters neurons is poorly understood. We found that BoNT/A enters neurons by binding to the synaptic vesicle protein SV2 (isoforms A, B, and C). Fragments of SV2 that harbor the toxin interaction domain inhibited BoNT/A from binding to neurons.

2025年1月1日 · Here we examined SV-2, the presynaptic protein involved in vesicle fusion with the neuronal presynaptic membrane, and the AChR as a post synaptic marker using α-bungarotoxin (α-BTX) as a labelling agent. Additionally, all neurons were labelled with a genetically encoded yellow fluorescent protein (YFP) to visualize neuronal cell bodies and axons.

2021年6月30日 · We observed a significant reduction in the number of BTX and SV2 puncta in cells expressing eGFP-FUS R514G (p < 0.05) and eGFP-FUS ΔNLS (p < 0.05) compared to eGFP-FUS WT (Fig. 4B).

To quantitatively assess the degree of colocalization between anti-SV2 and R-BTX, we examined the length of overlap between the two images along line intensity ...

BTX-A binds SV2 and the vesicle is recycled by the neuron and brought back into the cell, the BTX is said to be internalized. However, the light chain must still gain access from the recy-cled vesicle to the cytosol in order to perform its function. To do this, BTX-A undergoes a pH-dependent conformational change that causes a

We found that BoNT/A enters neurons by binding to the synaptic vesicle protein SV2 (isoforms A, B, and C). Fragments of SV2 that harbor the toxin interaction domain inhibited BoNT/A

BTX-A is a neurotoxin produced from the bacterium Clostidium botulinum. BTX-A binds to receptors on presynaptic cholinergic nerve terminals and blocks the release of acetylcholine. Intramuscular injections of BTX-A is used for focal spasticity and dystonia but also, for example, for overactive bladder, hyperhidrosis, and cosmetic procedures.

2021年12月8日 · 例如，突触前标签：sv2 或 nf，突触前颜色：r，突触后标签：btx，突触后颜色：g。 注意：对于专有文件格式，必须指示标签和相应的通道（C1、C2 或 C3）。

This meta-analysis suggests that treatment with BTX-A is effective and safe for neurogenic detrusor overactivity, and recommends using BTX-A with 300 U or with 200 U, as suitable dosage.