2018年2月23日 · Using a combination of live-cell lattice light-sheet microscopy, 3D structured illumination microscopy, correlative light electron microscopy, and electron cryotomography, we found that after BAK/BAX activation and cytochrome c loss, the mitochondrial network broke down and large BAK/BAX pores appeared in the outer membrane.

2017年4月1日 · In the mitochondria of apoptotic cells, Bax clusters into heterogeneous structures including arcs and rings that are associated with membrane pores. Bax shuttles between the cytosol and MOM in healthy cells and activates independently of BH3-only proteins when prosurvival Bcl-2s are downregulated.

2024年3月19日 · One hallmark of apoptosis is the oligomerization of BAX and BAK to form a pore in the mitochondrial outer membrane, which mediates the release of pro-apoptotic intermembrane space proteins into...

2024年6月3日 · We show that the increase in the unsaturation of lipids nearby the apoptotic pore strongly promotes the pore activity of BAX and BAK not only in vitro, but also at the organelle level and in...

2022年3月26日 · Specifically, BAX and BAK initiate MOMP by forming hetero- and homo-oligomeric pores in the outer mitochondrial membrane (OMM), in a process that is inhibited by anti-apoptotic proteins such as...

2022年3月3日 · BAX and BAK are key apoptosis regulators that mediate the decisive step of mitochondrial outer membrane permeabilization. However, the mechanism by which they assemble the apoptotic pore remains obscure.

2017年8月5日 · Apoptotic cell death via the mitochondrial pathway occurs in all vertebrate cells and requires the formation of pores in the mitochondrial outer membrane. Two Bcl-2 protein family members, Bak and Bax, form these pores during apoptosis, and how they do so has been investigated for the last two decades.

2023年6月2日 · Here, we show that Bax creates pores by extracting lipids from outer mitochondrial membrane mimics by formation of Bax/lipid clusters that are deposited on the membrane surface.

2022年3月3日 · BAX and BAK are key apoptosis regulators that mediate the decisive step of mitochondrial outer membrane permeabilization. However, the mechanism by which they assemble the apoptotic pore remains obscure. Here, we report that BAX and BAK present distinct oligomerization properties, with BAK organizin …

2017年6月1日 · How cytochrome C is released from the mitochondria to the cytosol via Bax oligomeric pores, a process which is required for apoptosis, is still a mystery. Based on experimentally measured...