BHQ™ Probes are traditional, linear, dual-labelled FRET probes, typically 20 to 30 bases in length, with a fluorophore and quencher covalently attached to the 5' and 3' ends, respectively. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridisation to its ...

Black Hole Quencher™ 1 (BHQ™-1) (with an absorption maximum of 534 nm) is absorbed at higher wavelengths than DABCYL and is directly superimposable with the emission maxima of FAM, TET and JOE. This provides a significant increase in both static and FRET quenching efficiencies, which makes it an excellent quencher for Molecular Beacons and ...

BHQplus™ Probes are compact, dual-labelled, hydrolysis probes for qPCR. This probe type features a 5' fluorescent dye and a 3' Black Hole Quencher™ (BHQ) with the unique BHQplus duplex stabilising chemistry to enhance specificity and mismatch discrimination.

BHQplus Probes are an advanced probe type with duplex-stabilising chemistry to allow for the design of shorter oligonucleotides with relatively high melting temperatures. BHQplus Probes are used to detect difficult targets, such as AT-rich regions or SNPs.

BHQplus probes are a new and advanced probe technology for qPCR that bring researchers many of the benefits of traditional MGB probes without their expense. As with MGB probes, BHQplus probes form highly stable duplexes with DNA targets allowing shorter probes to be used for hybridization-based assays.

BHQ dyes work through a combination of FRET and static quenching to enable researchers to avoid the residual background signal common to fluorescing quenchers such as TAMRA, or low signal to noise ratio. These quenchers can be paired with all common reporter dyes to construct efficiently quenched qPCR probes for multiplexing assays.

Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube.

The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye. What differentiates the BHQplus probe from other traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for detecting more ...

The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye. What differentiates the BHQplus probe from other traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for detecting more ...

BHQ® quenchers are the most popular and used quenchers for fluorescence quenched qPCR probes as they absorb a wide range of wavelengths from 480 nm to 730 nm. How to order. Why choose BHQ® quenchers? BHQ® patents for diagnostic and commercial use were lifted. BHQ® were engineered to be true black quenchers with no native fluorescence.