1998年3月20日 · First, the aqueous pore through a ribosome-free translocon is only 9–15 Å in diameter and is tightly sealed at its lumenal end by the nucleotide-dependent binding of BiP. …

2005年1月1日 · We have explored the molecular mechanisms governing BiP-mediated gating activity, including the coupling between gating and the BiP ATPase cycle, and the involvement …

1998年3月20日 · Since BiP is the gating protein that binds to and seals the lumenal end of the aqueous translocon pore, both immediately after ribosomal targeting to the ER membrane and …

Biophysical studies have suggested that BiP seals the lumenal face of the translocon during membrane protein integration (Hamman et al. 1998; Haigh and Johnson 2002). A second …

2000年9月15日 · The ribosome is responsible for sealing the cytoplasmic end of the translocon during cotranslational translocation and some stages of cotranslational integration (Johnson …

2002年1月21日 · Comparing integration intermediates prepared with intact, empty, or BiP-loaded microsomes revealed that the lumenal end of the translocon pore is closed by BiP in an ATP …

2002年1月1日 · We have shown in this study that BiP is required to seal the lumenal end of the aqueous translocon pore during the cotranslational integration of a nascent membrane protein, …

2002年2月1日 · Comparing integration intermediates prepared with intact, empty, or BiP-loaded microsomes revealed that the lumenal end of the translocon pore is closed by BiP in an ATP …

2000年9月15日 · The ribosome is responsible for sealing the cytoplasmic end of the translocon during cotranslational translocation and some stages of cotranslational integration (Johnson …

Extraction of soluble lumenal proteins from microsomes and reconstitution with purified proteins demonstrate, by fluorescence collisional quenching, that BiP seals the lumenal end of this …