1999年12月1日 · Recombinant BmK M1 was purified rapidly and efficiently by ion exchange and gel filtration chromatography to homogeneity, produced a single band on tricine–SDS–PAGE, and processed the homologous N-terminus.

Our results reveal large mutant-dependent differences that emphasize important and specific roles for the studied residues. By mutating single amino acids, we were able to redirect the alpha-like characteristics of BmK M1 (active on both mammals and insects) to either much higher mammal specificity or, in a few cases, total insect specificity.

BmK-M1 is a scorpion toxin, and is composed of 64 amino acids cross-linked by four disulfide bridges. BmK-M1 inhibits Na + channel and can be considered both as a cardiotoxin and a neurotoxin.

马氏钳蝎 ( Buthus martensii Karsch, 以下简称 BmK) 广泛分布于中国和东亚地区, 与非洲、南美等地可以致人死命的蝎神经毒素不同, BmK 具有较弱的神毒性, 事实上在中国从未有因蝎蛰造成死亡的报道. 相反, 全蝎作为名贵中药, 在中国已有上千年的历史, 对偏瘫、神经麻痹、癫痫、镇痛等神经疾患具有确切疗效. 因此对马氏钳蝎神经毒素结构-功能的研究有可能了解这些药物活性的生化和分子生物学基础. 关键词: 蝎神经毒素是存在于蝎尾毒液中的一组活性蛋白/多肽, 可概分为长链毒 …

To understand the respective roles of these disulfide bridges, a mutagenesis analysis for the four disulfide bonds, 12-63, 16-36, 22-46 and 26-48, of a representative toxin BmK M1 from the scorpion Buthus martensii Karsch was carried out. All cysteines were replaced by serine with double mutations.

1997年7月1日 · The cDNA library of venomous glands of the scorpion Buthus martensii Karsch (BmK) was constructed. A cDNA encoding a mammalian neurotoxin corresponding to the known α-type toxin, BmK M1, was amplified by polymerase chain reaction (PCR) and cloned, and its full-length sequence was determined.

2003年4月29日 · Scorpion alpha-neurotoxins can be classified into distinct subgroups according to their sequence and pharmacological properties. Using toxicity tests, binding studies, and electrophysiological recordings, BmK M1, a toxin from the Asian scorpion Buthus martensi Karsch, was experimentally identified a …

东亚钳蝎中哺乳动物毒素BmK M8是经典的α型毒素,而BmK M1和BmK M4则是α-like型毒素.为了进一步研究哺乳动物毒素中结构与功能的关系,针对东亚钳蝎哺乳动物毒素BmK M1中Face A和Site C中的5个位点设计了7个单点突变,利用酵母表达体系得到了7个重组的突变体,测定了它们 ...

2005年1月27日 · In this study, an effective yeast expression system was used to study the role of 14 N- and C-terminal residues from the α-like toxin BmK M1 from the Chinese scorpion Buthus martensii Karsch. With the use of site-directed mutagenesis, all of these residues were individually substituted by one or more amino acids, resulting in a total of 19 ...

2005年4月1日 · This study represents a thorough mapping and elucidation of three epitopes that underlie the molecular basis of the mammalian and insecticidal potency of the scorpion α‐like toxin, BmK M1 on voltage‐gated Na+ channels.