Obtained results indicated that BMSC SAM/P6 and BMSC BALB/c show the typical phenotype of mesenchymal stromal cells (CD44+, CD73+, CD90+) and do not express CD45. Further, BMSC SAM/P6 were characterized by deteriorated multipotency, decreased metabolic activity and increased apoptosis occurrence, accompanied by elevated oxidative stress and ...

2022年8月29日 · 1、bmsc用于治疗膝关节软骨损伤，患者疼痛显著降低，膝关节功能指数显著改善，而软骨损伤显著减少。 2、MSC可以抑制GVHD（移植物抗宿主反应），能够促进造血干细胞植入，降低细胞或器官移植后的免疫排斥反应。

骨髓间充质干细胞 （ BMSCs）是在哺乳动物骨髓基质中广泛存在的一类多能基质干细胞，BMSCs因为具有强大的治疗潜能已成为广大研究人员关注的焦点。 BMSCs属于 全能型间质干细胞，基于其各种特性，BMSCs广泛用于 抗肿瘤基因治疗 、 造血干细胞移植 、 组织损伤修复 和 自身免疫疾病。 现今，在分离、纯化大鼠BMSCs方面，可通过诸多途径来分离BMSCs，应用广泛的包括贴壁筛选、密度梯度离心与骨组织消化法。 近年来，还出现了新的方法，如 免疫磁珠分 …

2013年7月1日 · Results of the present study indicate that mouse BMSC cells are polyclonal and that the BMSC-like Sca-1+/CD45+/CD31+ subset was effective in directing cardiac differentiation and improving cardiac function in mice with myocardial infarct. Our in vivo findings warrant replication given the relatively small number of mice studied. Nevertheless ...

Representative expression of BMSC surface markers CD29, CD90, CD44, CD45, CD11 and CD34 as analyzed by flow cytometry. BMSCs were observed to hyperexpress CD29 (97.7%), CD90 (99.2%) and CD44...

Consistent with these studies, our data indicates PDGFRα + BMSC are exquisitely sensitive to high oxygen tension, undergoing rapid cell death as indicated by the high percentage of TUNEL positive cells when compared to CD45 + hematopoietic cells. BMSC cultured in 1% oxygen were characterized by a 10-fold reduction in programmed cell death when ...

2006年3月9日 · In this study, we showed that CD45 can be induced to present on the CD45 − MSCs by adding demethylating agent AZA to the culture medium. The demonstration of CD45 directly on the ‘fibroblast...

2021年5月13日 · The identification of these BMSCs was done by using flow cytometry for the detection of expressed surface antigens CD45, CD11B, CD44, and CD90. Using HCN2 and HCN4 genes cotransfected into BMSCs as group HCN2+HCN4 while myocardial induction solution was used to induced BMSC differentiation in the BMSC induction group.

The objective of this study was to screen mouse bone marrow mesenchymal stromal cells (BMSCs) according to expression of cardiac stem cell (CSC) surface antigens and to assess the effects of resulting BMSC-like subsets on cardiac function after injection in a …

Obtained results indicated that BMSC SAM/P6 and BMSC BALB/c show the typical phenotype of mesenchymal stromal cells (CD44+, CD73+, CD90+) and do not express CD45. Further, BMSC SAM/P6 were characterized by deteriorated multipotency, decreased metabolic activity and increased apoptosis occurrence, accompanied by elevated oxidative stress and ...