2023年4月6日 · Here, we show that matrix stiffness fine-tunes HSC niche factor expression by bone marrow stromal cells (BMSCs). Increased stiffness activates Yap/Taz signaling to promote BMSC expansion upon 2D culture, which is largely reversed by 3D culture in soft gelatin methacrylate hydrogels.

2023年4月7日 · 骨髓硬度可能通过影响 BMSC 中转录因子 Yap/Taz 的核定位，来调节 BMSC 增殖状态和 HSC 维持因子表达。 通过模拟最接近年轻骨髓硬度和微环境特征的 3D 培养系统，岳锐教授团队找到了 5% GelMA 的最优解，在该环境下共培养的 BMSCs 和 HSCs ，表现出了衰老标志物 …

造血干细胞（Hematopoietic stem cell，HSC）具有自我更新和分化为所有下游血细胞的能力，在整个生命周期中不断为机体提供新鲜血液。在衰老过程中，HSC会发生髓系分化偏好、淋系分化减少、造血重建能力受损和克隆…

2011年7月1日 · The expression of BMSC genes encoding functionally important proteins was compared with fibroblasts, HSC and hESC. The upregulated genes in BMSC compared with HSC and hESC included Hepatocyte growth factor (HGF), IL-6, VCAM1, chemokine (C-X-C motif) ligand (CXCL12), prostaglandin E synthase (PTGES), CD44 and CD59.

2023年4月6日 · Culture of BMSCs in 3D soft matrix induces the secretion of HSC niche factors by BMSCs in comparison to 2D culture; maintains HSC self-renewal, myelopoiesis, and lymphopoiesis; reverses aging hallmarks of HSCs; and restores their long-term multilineage reconstitution capacity.

Here, we show that matrix stiffness fine-tunes HSC niche factor expression. by bone marrow stromal cells (BMSCs). Increased stiffness activates Yap/Taz signaling to promote BMSC expansion upon 2D culture, which is largely reversed by …

2023年4月6日 · In situ atomic force microscopy analysis reveals that mouse bone marrow stiffens with age, which correlates with a compromised HSC niche. Taken together, this study highlights the biomechanical regulation of the HSC niche by BMSCs, which could be harnessed to engineer a soft bone marrow niche for HSC rejuvenation.

2023年9月1日 · A recent study published in Cell Stem Cell [1] showed that matrix stiffness critically regulates hematopoietic stem cell (HSC) niche, and successfully engineered a soft bone marrow (BM) organoid to maintain and rejuvenate HSCs ex vivo. In addition, BM stiffening was also identified as a novel aging hallmark of the hematopoietic system.

该研究发现基质硬度密切调控骨髓造血微环境，并利用柔软的甲基丙烯酰化水凝胶（GelMA）构建了BMSC与HSC的体外3D共培养体系。 该系统不仅可以促进HSC自我更新和淋系分化，还能显著逆转衰老HSC的各项功能指标。 此外，研究人员还揭示了骨髓硬化是血液系统衰老的新型标志物。 这一重要研究成果为衰老HSC年轻化及衰老相关疾病治疗提供了新思路和新策略。 研究人员首先利用流变仪测量了年轻小鼠骨髓块的硬度，发现其相当柔软（约为200Pa）。

2023年4月8日 · 该研究表明基质硬度微调骨髓基质细胞(BMSCs)的HSC小生境因子表达。增加的硬度激活Yap/Taz信号通路，促进BMSC在2D培养中的扩张，这在很大程度上被3D培养中的软明胶甲基丙烯酸酯水凝胶逆转。