2011年9月15日 · The lack of an established protocol for scanning electron microscopy (SEM) studies on stem cells differentiating into adipogenic lineage led us to develop a protocol for the preparation of differentiated adult bone marrow-derived mesenchymal stem cells (BMSC) for …

2022年11月1日 · In detail, BMSC-EVs suppressed the aberrant proliferation, high collagen production capacity, and activation of fibrotic pathways in TGF-β-stimulated fibroblasts in vitro. Besides, in vivo, BMSC-EVs reduced cell infiltration, reduced fibrotic tissue in the shoulder capsule, and improved shoulder mobility. In addition, via exosomal small RNA ...

2022年7月28日 · TGF-β1 stimulated bone marrow mesenchymal stem cells (BMSCs) to promote cell migration at lesion sites in vitro and in vivo. The mRNA and protein levels of several migration-related genes (N...

2020年5月1日 · Here, the response of bone marrow stem cells (BMSCs) to electrospun gradient nanofibrous scaffolds was investigated to demonstrate their potential capabilities for interfacial tissue regeneration. The electrospun scaffolds showed gradient distribution of BMP-2/nanoHA contents and the fiber orientations gradually changed from random to align.

2025年1月9日 · This study aims to elucidate the communication between BMSC-derived exosomal miRNA and triple-negative breast cancer (TNBC) in a hypoxic environment. Exosomes were isolated via ultracentrifugation and identified using scanning electron microscopy (SEM), nanoparticle tracking analysis (NTA) and western blot.

2021年2月9日 · We show that SFM tested supports the growth and expansion of BM-MSCs even at low seeding density and may serve as possible replacement for animal-derived serum. Mesenchymal stromal cells (MSCs)...

骨髓间充质干细胞 （ BMSCs）是在哺乳动物骨髓基质中广泛存在的一类多能基质干细胞，BMSCs因为具有强大的治疗潜能已成为广大研究人员关注的焦点。 BMSCs属于 全能型间质干细胞，基于其各种特性，BMSCs广泛用于 抗肿瘤基因治疗 、 造血干细胞移植 、 组织损伤修复 和 自身免疫疾病。 现今，在分离、纯化大鼠BMSCs方面，可通过诸多途径来分离BMSCs，应用广泛的包括贴壁筛选、密度梯度离心与骨组织消化法。 近年来，还出现了新的方法，如 免疫磁珠分 …

2010年2月8日 · The aim of this study was to investigate in vitro the role of human bone marrow–derived mesenchymal stromal cells (BMSC) and active osteoblasts in control of migration, lodgment, and proliferation of HSCs.

2019年11月14日 · Our data demonstrate that BMSC progenitors possess a distinct metabolic program and are poised to respond to exogenous metabolic cues that regulate their differentiation fate.

2022年1月2日 · Bone marrow mesenchymal stromal cells (BMSCs), identified as pericytes comprising the hematopoietic niche, are a group of heterogeneous cells composed of multipotent stem cells, including osteochondral and adipocyte progenitors. Nevertheless, the identification and classification are still controversial, which limits their application.