2023年8月1日 · New vascular structures with an inner layer of endothelial cells and an outer layer of mature and functional pericytes are found by the activation via VEGF/VEGFR2/ERK signaling. Co-culturing EC-BMSC within VEGF/HA-DN hydrogels is promising to achieve early and enhanced angiogenesis in tissue engineering applications.

2024年4月9日 · EC-derived cell membrane vesicles (EC-CMVs) also contained BMP2 and VEGF derived from their parental cells. BMSC-CMVs enhanced tube formation and migration ability of hUVECs, while EC-CMVs...

2021年2月1日 · Immunofluorescence staining and hematoxylin-eosin (H&E) staining showed that bone marrow mesenchymal stem cell (BMSC) was embedded in fibrin gel. Furthermore, vascular endothelial growth factor (VEGF) was used to induce BMSC to differentiate into CD31+ and vWF+ endothelial cell (EC) in fibrin gel.

2021年1月19日 · Here, we probed the potential of bone marrow stem cells (BMSCs) engineered with chemically modified mRNAs (modRNA) encoding the hBMP-2 and VEGF-A gene to therapeutically heal bone. Induction of...

2020年7月2日 · In this study, we show that sustained VEGF over-expression in clinical-size osteogenic grafts by genetically modified rabbit BMSC was effective to significantly: (1) increase the depth of tissue ingrowth; (2) improve the long-term survival of seeded progenitors and the formation of mineralized tissue; (3) support the establishment of a ...

2013年7月1日 · Vascular endothelial growth factor (VEGF) is the master regulator of vascular growth. We investigated a cell-based gene therapy approach to generate osteogenic grafts with an increased vascularization potential in an ectopic nude rat model in vivo, by genetically modifying human bone marrow-derived stromal/stem cells (BMSC) to express rat VEGF.

通过共同培养MSC和EPC，研究了MSCs分泌的血管内皮生长因子（VEGF）通过旁分泌机制对EPC分化的影响。 逆转录-定量聚合酶链反应和蛋白质印迹分析用于检测目的基因和蛋白质的表达。 本结果表明，将EPC与MSC共培养可增强分化簇31和von Willebrand因子的表达，它们是内皮表型的特异性标志物，因此表明MSC可能在体外影响EPC的内皮分化。 VEGF似乎对该过程至关重要。 这些发现对于理解MSC和EPC之间的生物学相互作用以及开发基于干细胞的疗法在治疗缺 …

The highest degree of osteogenesis was found when the carrier was loaded with BMSC (group 3), whereas VEGF-transfected cells led to the highest vascularization and fastest resorption of the bone substitute. Additionally, VEGF-transfected BMSC …

2023年6月1日 · Co-existence of ECs and BMSCs with the VEGF within the hydrogel-based extracellular matrix (ECM) plays a key role in mediating the formation of a mature vascular structure with endothelium and...

2018年10月30日 · VEGF-BMSCs or BMSCs was transplanted via tail vein IV injection. Endometrial thickness, morphology, and pinopodes were assessed by hematoxylin and eosin (HE) staining and scanning electron microscope (SEM). The proteins and mRNAs expressions of markers for endometrial cells and endometrial receptivity were measured after treatment.