Immunohistochemistry (IHC) is the process of identifying proteins in tissue sections by incubating the sample with antibodies specific to the protein of interest, and then visualizing the bound antibody using a chromogen. Unlike in situ hybridization, which identifies gene transcripts in …

Immunostaining is the process of identifying proteins in tissue sections by incubating the sample with antibodies specific to the protein of interest, then visualizing the bound antibody using a chromogen (immunohistochemistry or IHC) or fluorescence (immunofluorescence or IF).

2015年5月15日 · Bone marrow (BM) tissue biopsy evaluation, including trephine biopsy and clot section, is an integral part of BM investigation and is often followed by ancillary studies, in particular immunohistochemistry (IHC). IHC provides in situ coupling of morphological assessment and immunophenotype.

2020年11月12日 · Immunophenotyping is an important part of the integrated haematopathologic diagnostics of bone marrow (BM) samples. Integrated diagnosis should include clinical information, peripheral blood (PB) and BM smear cytology, flow cytometry (FCM) of BM aspirate, BM trephine biopsy (BMB) morphology, BMB immunohistochemistry (IHC) and cytogenetic ...

2014年1月1日 · Here we describe a detailed protocol for IHC analysis of bone and cartilage, addressing specific issues associated with staining of hard and matrix-rich tissues. In both clinical and research studies, histology-based methods are critical for describing phenotypes in patients and in experimental organisms. There are four basic parts to IHC:

Immunohistochemistry (IHC) is an important auxiliary method for pathologists as it specifically visualizes distribution and amount of a certain molecule in the tissue using specific antigen-antibody reaction.

Samples from rat femoral and human vertebral bone were processed with a detailed and effective IHC protocol summarized here. First, a novel antigen retrieval (AR) method of hyaluronidase combined pepsin predigestion (H+P) was established and the optimal concentration and pH value for AR of bone specimens were determined.

2021年7月1日 · In this study, we optimized selected steps of in situ hybridization (ISH), immunohistochemistry (IHC), and immunofluorescence (IF) for formalin fixed and decalcified skeletal tissues.

Bone marrow (BM) tissue biopsy evaluation, including trephine biopsy and clot section, is an integral part of BM investigation and is often followed by ancillary studies, in particular immunohisto-chemistry (IHC). IHC provides in situ coupling of morphological assessment and immunophenotype.

CD3 positive cells in bone marrow trephine biopsy using IHC staining method. Interpreting IHC stains requires a keen eye and a thorough understanding of the specific antibody, target antigen, and tissue context. Here’s a breakdown of what to expect and how to decode the visual clues: Staining Patterns