2022年10月26日 · LC3 lipidation aids in autophagosome trafficking and directs autophagosome/lysosome fusion events as well as other functions including cargo selection in canonical autophagy. In LAP, LC3 lipidation occurs on the outer face of the phagosome membrane following full phagosome closure.

LC3-I to LC3-II conversion can be readily assessed in Western blots due to the distinct electrophoretic mobility of these isoforms, or through the LC3 dots that can be detected by immunofluorescence. A high ratio of LC3-I to LC3-II is common in neuronal cells and only a slight increase in LC3-II occurs on inducing autophagy by nutrient ...

Microtubule-associated protein 1A/1B-light chain 3 (LC3) is a soluble protein with a molecular mass of approximately 17 kDa that is distributed ubiquitously in mammalian tissues and cultured cells. During autophagy, autophagosomes engulf cytoplasmic components, including cytosolic proteins and organ …

By immunohistochemistry, we studied the expression of microtubule-associated protein 1 light chain 3 (LC3), an autophagosome marker, in 5 NHD and 12 control brains. In all NHD brains, Nogo-A-positive, CNPase-positive oligodendrocytes surviving in the non-demyelinated white matter intensely expressed LC3.

LDN-214117 and LRRK2-in-1 were the best two of sixteen transforming growth factor-beta1 (TGF-β) receptor and autophagy mediators to decrease Postn mRNA. These data led us to conclude that LC3 accelerated motoneuron secretion of POSTN and aggravated the RIBE in the lung after brain irradiation.

2023年5月29日 · Our results reveal a role of glial LAP in the clearance of neuronal debris in vivo, with potential implications for the recovery of the injured nervous system. Neural injuries, stroke and...

LC3-associated phagocytosis (LAP) is one such novel function for autophagy proteins and is a contributor to immune regulation and inflammatory responses across various cell and tissue types.

2019年7月25日 · We have identified a novel non-canonical function of several autophagy proteins in the conjugation of LC3 to Rab5 +, clathrin + endosomes containing β-amyloid in a process of LC3-associated endocytosis (LANDO). We found that LANDO in microglia is a critical regulator of immune-mediated aggregate removal and microglial activation in a murine ...

2019年7月25日 · We have identified a novel non-canonical function of several autophagy proteins in the conjugation of LC3 to Rab5 +, clathrin + endosomes containing β-amyloid in a process of LC3-associated endocytosis (LANDO). We found that LANDO in microglia is a critical regulator of immune-mediated aggregate removal and microglial activation in a murine ...

Macroautophagy (canonical autophagy) and LC3-associated phagocytosis (LAP) are lysosome-dependent catabolic processes involved in maintaining cellular homeostasis. Becn1 regulates both canonical autophagy and LAP (also termed as non-canonical autophagy), each of which has been implicated in the regulation of inflammation.