
BD Pharmingen™ Bromodeoxyuridine (BrdU) - BD Biosciences
Bromo-deoxyuridine (BrdU) is a thymidine analog that is used in cell proliferation studies. BrdU in culture is incorporated into the DNA during DNA synthesis. Cellular incorporation of BrdU can …
Many high-resolution studies of this type are possible with the use of the BD Pharmingen BrdU Flow Kit and other selected flow cytometry reagents. The kit ensures consistent results by …
BD Pharmingen™ FITC BrdU Flow Kit - BD Biosciences
Keep the fluorochrome-conjugated anti-BrdU antibody and 7-AAD protected from light. Unused portions of 1X BD Cytofix/Cytoperm Buffer can be stored at 2 to 8°C. BrdU and DNase.
实验中所用的BD LSRFortessa 流式细胞分析仪,配有355 nm/405 nm/488 nm/561 nm/638 nm 五根激光器。根据细胞仪配置,BrdU 的信号由Alexa FluorTM488 荧光 信号标记,用488 nm 激 …
【罗工宝典第18式】BrdU的检测攻略 - 知乎 - 知乎专栏
5-溴脱氧尿嘧啶核苷 (5-Bromo-2-deoxyUridine,BrdU),一种类似于DNA前体胸苷激酶的物质,在S期可替代 胸腺嘧啶核苷 选择性结合到细胞DNA中,当细胞处于DNA合成期(S期),而 …
BD APC BrdU Flow Kit, 50 tests - Fisher Sci
Provides reagents for immunofluorescent BrdU staining with a protocol, compatible with the use of additional fluorescent antibodies specific for other cellular molecules. Used with fluorescent …
做实验 | 一文掌握 BrdU标记法检测细胞增殖 - 知乎
细胞经过固定和变性处理后,可用 免疫学方法 检测DNA中BrdU的含量(如采用鼠抗BrdU单克隆抗体特异识别BrdU,再采用 辣根过氧化酶 标记的 山羊抗鼠IgG二抗 标记,最后用比色法或荧光 …
细胞增殖的流式检测(BrdU 法) - 哔哩哔哩
2024年11月7日 · 实验方法概述 :在检测细胞增殖的多种方法中,直接测定DNA合成是一种敏感并高精度的分析方法;5-溴-脱氧尿嘧啶核苷 (Bromodeoxyuridine,BrdU),是一种胸腺嘧啶核苷 …
BrdU,英文全称5-bromo-2′-deoxyuridine,中文全称5-溴-2’-脱氧尿苷,是一种合成的胸苷溴化类似 物,在S期可替代胸苷选择性插入细胞DNA。 BrdU通常和广泛用于测定DNA合成和标记分裂 …
BD Pharmingen™ Purified Mouse Anti- BrdU - BD Biosciences
Investigators can identify cycling cells in an asynchronous cell population and determine cell cycle kinetics by detecting incorporated BrdU. The 3D4 monoclonal antibody reacts with BrdU, but …