直接测定DNA合成是细胞增殖检测的最准确方法之一，是测定物质毒性、评估药物安全评价、细胞健康的基本方法，其中常用的方法有 BrdU标记法 。 >>> BrdU检测原理

Incorporation of the thymidine analog 5′-bromo-2′-deoxyuridine (BrdU) has been established as a popular assay for determining cell proliferation rates in a wide variety of species, ranging from plants to mammalian cells (Cecchini et al. 2012, Nagar et al. 2002).

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical structure similar to thymidine. BrdU is commonly used to study cell proliferation in living tissues [1] and has been studied as a radiosensitizer [2] and diagnostic tool in people with cancer. [3]

2023年1月30日 · BrdU (bromodeoxyuridine or 5-bromo-2'-deoxyuridine) is an analog of the nucleoside thymidine used in the BrdU assay to identify proliferating cells. BrdU labeling can be performed in vitro for cell lines and primary cell cultures …

BrdU是一种胸腺嘧啶核苷类似物。 它也能够在细胞增殖时期代替胸腺嘧啶（T）渗入正在复制的DNA分子，通过基于EdU与Apollo荧光染料的特异性反应检测DNA复制活性，通过检测EdU标记便能准确地反映细胞的增殖情况。

Step-by-step protocol for the use of BrdU (5-Bromo-2´-Deoxyuridine) to detect DNA synthesis using a fluorescence microscope.

2024年10月8日 · BrdU (5-bromo-2'-deoxyuridine) is a widely used tool in molecular and cell biology research for tracking cell proliferation and DNA synthesis. As a synthetic nucleoside analog, BrdU is incorporated into the DNA of replicating cells during the S phase, allowing scientists to label and monitor newly synthesized DNA.

Cell proliferation assays are used for a large variety of applications in the life sciences. This unit describes a flow-cytometry-based method that uses BrdU labeling in conjunction with multiple fluorescently labeled cell surface markers, allowing extensive phenotypic characterization of …

Immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool for studying the dynamics of normal and tumor cell proliferation. Labeling tumor cells with BrdU, either in vitro or in vivo, followed by detection with specific anti-BrdU monoclonal antibodies, is an accurate and comprehensive method for ...

Bromo-deoxyuridine (BrdU) incorporation into replicating DNA is a commonly used and highly reproducible method to detect cell division history. Here, we describe a protocol for BrdU incorporation analysis in hematopoietic stem and progenitor cells that can provide a sensitive measure of cell division even in rare cell populations.