2023年1月30日 · Protocol for identifying proliferating cells using BrdU. BrdU (bromodeoxyuridine or 5-bromo-2'-deoxyuridine) is an analog of the nucleoside thymidine used in the BrdU assay to identify proliferating cells. BrdU labeling can be performed in vitro for cell lines and primary cell cultures or in vivo for labeling cells within a living animal.

Step-by-step protocol for the use of BrdU (5-Bromo-2´-Deoxyuridine) to detect DNA synthesis using a fluorescence microscope.

BrdU 染色试剂盒 包含通过流式细胞术来鉴定并检测细胞增殖所需的试剂和缓冲液。 用 5-溴-2’-脱氧尿嘧啶核苷（BrdU）培养处于分裂周期的细胞。 BrdU 是胸苷的一种合成胸腺嘧啶类似物， …

细胞经过固定和变性处理后，可用 免疫学方法 检测DNA中BrdU的含量 (如采用鼠抗BrdU单克隆抗体特异识别BrdU,再采用 辣根过氧化酶 标记的 山羊抗鼠IgG二抗 标记，最后用比色法或荧光的方法进行定量测定)，从而判断细胞的增殖能力。

The BrdU Staining Kits for Flow Cytometry contains the necessary reagents and buffers for identifying and examining proliferating cells by flow cytometric analysis. Cycling cells are incubated with 5-bromo-2’- deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis.

BrdU antibody staining protocols for flow cytometry and immunohistology. Steps include preparation of reagents and methods for staining with rabbit and mouse anti-BrdU antibodies such as Mouse Anti-BrdU Antibody, clone Bu20a (MCA2483) …

BrdU (Bromodeoxyuridine / 5-bromo-2'-deoxyuridine) is an analog of the nucleoside thymidine normally used in the BrdU assay to identify proliferating cells. BrdU labeling can be performed …

With the availability of new highly specific anti-BrdU antibodies, the BrdU labeling technique can be combined with click chemistry detection for a simplified method of dual pulse labeling.

细胞增殖可使用胸苷类似物 BrdU（5-溴-2'-脱氧尿苷）进行测定，方法是将胸苷类似物 BrdU掺入新合成 DNA，并随后使用抗 BrdU 抗体进行检测。 Invitrogen Alexa Fluor 标记二抗 （如 货 …

Detection of BrdU is commonly performed in combination with total DNA staining. When performing cell cycle analysis, propidium iodide (PI) tends to be the DNA stain of choice (Figure 3).