2016年8月1日 · Among several subunits comprising HAT complexes, scaffolding proteins are critical for proper enzymatic activity. The scaffold protein BRPF2 (also called BRD1), a member of the BRPF family, links the MYST family of HATs with ING5 and EAF6, helping to recruit the proteins to specific target gene(s) and providing HAT histone tail specificity [4], [8], [29], [30], [31].

Our crystal structure of the BRPF2 PWWP domain in complex with a 12-mer dsDNA reveals that the PWWP domain interacts with dsDNA by binding to its major groove, instead of the minor groove observed in the HDGF family of PWWP domains. Our study indicates that PWWP domains could bind to dsDNA in different modes.

2017年6月2日 · BRPF2 has been shown to be important for the HAT activity of HBO1 toward H3K14. Here we demonstrated that BRPF2 can regulate the HAT activity of HBO1 toward free H3 and H4, and nucleosomal H3. Particularly, a short N-terminal region of BRPF2 is sufficient for binding to HBO1 and can potentiate its activity toward H3K14. The crystal structure of ...

2011年9月1日 · The histone acetyltransferases (HATs) of the MYST family include TIP60, HBO1, MOZ/MORF, and MOF and function in multisubunit protein complexes. Bromodomain-containing protein 1 (BRD1), also known as BRPF2, has been considered a subunit of the MOZ/MORF H3 HAT complex based on analogy with BRPF1 and BRPF3.

2021年3月1日 · Structural comparison of our BRPF2-dsDNA complex with the LEDGF-nucleosome complex structure (PDB ID: 6S01) indicated that the PWWP domain could superimpose very well, especially for the β-barrel subdomain, however there are some significant differences (Fig. 6 A) [21]. Firstly, the PWWP domains bind to different parts of dsDNA.

2017年4月10日 · 另外，体内实验结果也证明了brpf2的n端区域是hbo1-brpf2相互作用的重要位点，同时该区域也能够在一定程度上辅助该复合物结合核小体。 这些研究结果不仅揭示了BRPF2调节HBO1酶活的分子基础，而且也为进一步阐明其它组蛋白乙酰转移酶复合物的调节机制提供了 ...

2022年9月6日 · Moreover, the BRPF2 bromodomain could enrich H4K5ac mark-bearing mononucleosomes compared to other acetylated H4 marks. Consistent with this, ChIP-seq analysis revealed that BRPF2 strongly co-localizes with HBO1 at histone H4K5ac and H4K8ac marks near the transcription start sites in the genome. Our study provides novel insights into …

As expected, BRPF2 (1–205) can bind tightly to the HBO1 MYST domain. Intriguingly, BRPF2 (1–80) and BRPF2 (31–80) can also bind to HBO1 comparable to BRPF2 (1–205), whereas BRPF2 (81–205) and BRPF2 (1–30) cannot, indicating that the N-terminal region of BRPF2 encompassing residues 31–80 is sufficient to bind to the HBO1 MYST domain.

结果表明BRPF2是BRPF1的一种新型转录本(GenBank登录号：DQ288860); 在小鼠肝、胚胎、附睾、睾丸、卵巢和骨骼肌中均检测到 BRPF1和BRPF2两个转录本, 其中以BRPF1为主要的转录本; 而在小鼠脾中, 仅检测到BRPF2的转录本; BRPF1编码1246个氨基酸残基, 其编码的蛋白质分子量 …

2017年5月5日 · For BRPF2 and TAF1, experiments were performed in buffer (20 mM Hepes pH 7.5, 100 mM NaCl) on a VP-ITC microcalorimeter (MicroCal) at 20 °C. For CREBBP and TAF1L, experiments were done at 15 °C using a buffer containing 20 mM Hepes pH 7.5, 150 mM NaCl, and 0.5 mM TCEP. Protein concentrations were between 200 and 240 μM, and the compound ...