Bovine viral diarrhea virus (BVDV) membrane-anchored type I glycoprotein E2 is an ∼53-kDa immunodominant glycoprotein inducing the production of neutralizing antibodies in the animal host after natural infection or following immunization with live or killed vaccines.

摘要：旨在利用悬浮培养CHO细胞表达系统制备牛病毒性腹泻病毒 (bovine viral diarrhea virus, BVDV)E2蛋白，并鉴定纯化E2蛋白的免疫原性。 本研究以BVDV-1 NADL株基因序列为基础，构建BVDV E2蛋白的重组真核表达质粒pcDNA3.1-BVDV-E2，转染经悬浮培养的CHO细胞进行分泌表达，收集细胞培养上清，进行亲和层析法纯化，SDS-PAGE电泳鉴定蛋白表达和纯化，Western blot鉴定与His抗体和BVDV阳性血清反应性，利用纯化蛋白免疫新西兰大白兔，用细胞间接免 …

基于bvdv e2蛋白在宿主免疫反应中的重要地位，系统研究基因突变对同义密码子使用模式以及蛋白结构域正确形成的影响、合理构建多表位e2抗原，对采用e2蛋白作为核心组件的bvdv基因工程疫苗十分重要。

2021年5月14日 · The BVDV-E2 protein plays important role in viral infection and pathogenesis. We mapped some important potential neutralizing epitopes among some BVDV genomes especially the E2 protein. These novel epitopes could be promising targets against the currently circulating strains of BVDV.

The envelope of bovine viral diarrhea virus (BVDV) contains the glycoproteins Erns, E1 and E2. Complementation of a recombinant vesicular stomatitis virus (VSV) with BVDV glycoproteins resulted in infectious pseudotyped viruses. To elucidate the specific role of each of the single envelope glycoprot …

2023年1月6日 · Here, we report the development of a BVDV subunit vaccine by (i) the expression of a secreted form of a recombinant E2 glycoprotein using BHK21 cells and (ii) determination of the immune...

2014年12月5日 · E2 glycoprotein is a major structural component of the BVDV virion and plays a key role in viral attachment to host cells and inducing immune responses against viral infection. In order to gain detailed information of the E2 coding region of BVDV circulating in China, 46 positive samples were tested by RT-PCR for the E2 coding region.

Bovine viral diarrhea virus (BVDV) is a significant immunosuppressive pathogen that has a major impact on the global cattle industry. Research efforts are currently focused on the envelope glycoprotein E2 of BVDV to improve immune responses. However, the full-length E2 protein is not ideal as an imm …

2014年12月5日 · E2 glycoprotein is a major structural component of the BVDV virion and plays a key role in viral attachment to host cells and inducing immune responses against viral infection. In order to gain detailed information of the E2 coding region of BVDV circulating in China, 46 positive samples were tested by RT-PCR for the E2 coding region.

2000年8月10日 · The E2 glycoprotein of the bovine diarrhea disease virus (BVDV, genus Pestivirus), a major pathogen of cattle having world-wide economic impact, is the main target of neutralizing antibodies in BVDV-infected hosts.