Bovine viral diarrhea virus (BVDV), the prototypic pestivirus, possesses a positive-strand RNA genome with a single large open reading frame (ORF) encoding about 4000 amino acids. We have endeavored to elucidate the mechanisms involved in protein biogenesis by this pestivirus. Here, we present our s …

p80蛋白是BVDV非结构蛋白之一,在瘟病毒属中是一种突变率很低的非常保守的免疫优势蛋白,具有重要的诊断价值.本研究利用原核表达系统,成功诱导表达出p80重组蛋白,免疫印迹表明其具有良好的反应性.利用浓缩的BVDV病毒液免疫6~8周龄Balb/c小鼠,取免疫小鼠的脾细胞 ...

ID Screen® BVD p80 Antigen Capture ELISA Sandwich ELISA for the detection of the BVDV/ BDV P80 (NSP2-3) protein in serum, plasma, peripheral blood mononuclear leukocytes, tissue extracts or nasal swab samples from cattle, sheep, goats or other susceptible species

Competitive ELISA for the detection of Bovine Viral Diarrhoea / Mucosal Disease / Border Disease anti-p80-125 (anti-NSP2-3) antibodies in serum, plasma and milk from cattle, sheep, goats and other susceptible species

IDEXX BVDV p80 抗体检测试剂盒是一款阻断 ELISA 产品。 本试剂盒能用于多种不同类型样品：可检测牛血清、血浆及牛奶样品中的BVDV抗体；绵羊单份血清和5头份以下的混合血清中的边界病病毒抗体。 牛病毒性腹泻病毒（BVDV）可抑制母牛的免疫系统，从而使其易受多种其它传染病的侵袭。 这就是为什么BVDV是世界上代价最高的牛类疾病之一，由于牛群生产力、健康和繁殖效率的降低，每头牛损失 $15 至 $88 不等。 控制BVDV需要检出并清除传染源，因为持续性感 …

2019年7月10日 · 本研究用大肠杆菌对 BVDV的p80蛋白进行 的pET30a载体中，挑选阳性菌落，提取重组质粒并 了表达，制备了8株针对p80的单克隆抗体（MAb）， 酶切鉴定后进行序列测定，取鉴定正确的重组质粒 为BVDV的检测提供了技术支持。

2016年8月22日 · 利用纯化的重组蛋白 p80 作为检测抗原的间接 ELISA 筛选阳性杂交瘤细胞，经过 3 次亚克隆后对获得的杂交瘤细胞进行间接免疫荧光 (IFA)和抗体亚类鉴定及腹水的制备和效价的测定。 结果表明:重组 p80 蛋白具有很好的反应性;获得 8 株稳定分泌针对 BVDV p80 蛋白的杂交瘤细胞株，分别为 3E3、2A4、8H9、3G3、2B3、3G2、2G2 和 4A11;8 株杂交瘤细胞株接种 Balb/c 小鼠制备腹水，采用 BVDV 作为包被原的间接 ELISA 方法测得其效价为 1 ×10 5 ～1 ×10 7 ;8 …

The pestivirus bovine viral diarrhea virus (BVDV) p80 protein (referred to here as the NS3 protein) contains amino acid sequence motifs predictive of three enzymatic activities: serine proteinase, nucleoside triphosphatase, and RNA helicase.

Monoclonal antibodies reactive to the bovine viral diarrhea virus (BVDV) protein gp53 were produced and characterized. These antibodies and our panel of anti-p80/125 monoclonal antibodies were tested for their cross-reactivity with 11 different North American and European (Danish) BVDV strains and i …

The IDEXX BVDV p80 Ab Test is a blocking ELISA. It can be used on different samples: bovine serum and plasma, bovine milk samples for the detection of specific antibodies directed to bovine viral diarrhea virus, and ovine individual sera and pools of up to five sera for the detection of specific antibodies directed to the border disease virus.