Efficient site-specific integration of large genes in ... - Nature
2024年6月10日 · In single-transfection experiments at safe-harbour and therapeutically relevant sites, PASSIGE with eeBxb1 led to an average targeted-gene-integration efficiencies of 23% …
A novel Bxb1 integrase RMCE system for high fidelity site-specific ...
We built and characterized a novel recombinase mediated cassette exchange (RMCE) system using Bxb1 integrase and compared it to the commonly used Flp/FRT RMCE system.
Efficient targeted transgenesis of large donor DNA into ... - Nature
2022年3月31日 · Bxb1 Int-mediated recombination uses a minimal attachment site of 48 bp attP (attachment Phage) and a cognate 38 bp site attB (attachment Bacterial) between the donor …
The Bxb1 recombination system demonstrates heritable …
2012年3月21日 · The mycobacteriophage large serine recombinase Bxb1 catalyzes site-specific recombination between its corresponding attP and attB recognition sites. Previously, we and …
Bxb1 integrase serves as a highly efficient DNA recombinase in …
2017年1月1日 · Here, we introduce Bxb1 integrase as a more efficient candidate for joining multiple DNA molecules. Bxb1 integrase-mediated highly efficient metabolite pathway …
Accuracy and efficiency define Bxb1 integrase as the best of …
2013年10月20日 · Bxb1 integrase yielded approximately two-fold more recombinants and displayed about two fold less damage to the recombination sites than the next best …
Engineered Bxb1 variants improve integrase activity and fidelity
2024年10月21日 · To address these issues, we developed Bxb1 variants that demonstrate increased specificity and potency in vitro and have engineered stabilized Bxb1 variants that …
Mammalian Genomic Manipulation with Orthogonal Bxb1 DNA …
2023年11月3日 · Here, we show that a point mutation (Bxb1-GA) in Bxb1 target sites significantly increases Bxb1-mediated integration efficiency at the Rosa26 locus in Chinese hamster ovary …
Bxb1 Integrase: Mechanism of Large Cargo Integration
2 天之前 · Bxb1 integrase, a serine recombinase from mycobacteriophage Bxb1, is widely used for precise genetic modifications. Its efficiency in site-specific recombination allows for the …
Mammalian Genomic Manipulation with Orthogonal Bxb1 DNA …
2023年11月17日 · Here, we harnessed two orthogonal Bxb1 recombinase sites to enable alternative avenues for using mammalian synthetic biology to characterize transgenic protein …
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