The liquid PRF that was directly collected from the buffy-coat layer following L-PRF protocols was referred to as concentrated PRF (C-PRF). Results: The i-PRF protocol typically yielded a 2- to 3-fold increase in platelets and a l.5-fold increase in leukocyte concentration from the 1- to 1.2-mL plasma layer compared to baseline concentrations ...

2019年12月2日 · While conventional i-PRF protocols increase platelet yield by 2-3-fold and leukocyte yield by 50%, we convincingly demonstrated the ability to concentrate platelets and leukocytes over 10-fold by harvesting the 0.3–0.5 mL of …

Platelet-rich fibrin (PRF) represents second generation of platelet concentrates, which has gained increasing awareness in recent years for regenerative procedures.

Ideal platelet yield, whereas preparing liquid PRF preparation can be achieved using the C-PRF protocol (700 g for 8 min) using PET plastic vacutainers in a horizontal centrifuge. Steps of preparation for the traditional I-PRF (60 g for 3 min) and the newer C-PRF (700 g for 8 min) have been shown in Figures 9 and 10 respectively.

We therefore proposed harvesting this 0.3- to 0.5-mL layer directly above the red blood cell corpuscle layer as liquid C-PRF. In general, i-PRF was able to increase platelet numbers by ~ 250%, whereas a 12001700% increase in platelet numbers could easily be achieved by.

根据l-prf协议直接从血沉棕黄层中收集的液体prf称为浓缩prf（c-prf）。 结果与全血中的基线浓度相比，i-PRF方案通常使血小板从1-mL到1.2mL血浆层增加2到3倍，使白细胞浓度增加1.5倍。

Liquid platelet-rich fibrin (PRF) can be prepared by high centrifugation forces separating the blood into a platelet-poor plasma (PPP) layer and a cell-rich buffy coat layer, termed concentrated PRF (C-PRF). Heating the liquid PPP was recently ...

C-PRF is a novel technique to isolate liquid PRF directly from buffy coat layer above the red cell layer by using L-PRF protocols, it has 10-fold increase in platelet and leukocyte yields...

2021年1月15日 · The final and third protocol is that of C-PRF, where cells are purposefully accumulated specifically toward the buffy coat layer using faster spin protocols.

2020年8月1日 · C-PRF is a novel technique to isolate liquid PRF directly from buffy coat layer above the red cell layer by using L-PRF protocols, it has 10-fold increase in platelet and leukocyte yields...