2016年11月30日 · Here, we describe methodologies pertaining to a well-characterized model of cancer cachexia: mice bearing the C26 carcinoma 2-4. Although this model is heavily used in cachexia research, different approaches make reproducibility a potential issue.

C26 tumor-bearing mice are a commonly used experimental model of cancer cachexia 22-24. This model closely resembles the human disease, showing reductions in body, muscle, and fat mass, as well as muscle fiber atrophy and increased expression of inflammatory genes and ubiquitin ligases consistent with marked protein hypercatabolism 2,5 .

2024年11月1日 · The animal model of C26 cachexia is modeled by subcutaneous inoculation of C26 cells into BALB/c mice unilaterally at a typical inoculation rate of 5 × 10 5 –1 × 10 6 cells per mouse. 1213 The severity of C26-induced cachexia depends on the number of cell passages, the inoculation volume, and the injection site. 23,24 Anorexia does not occur whe...

2021年11月13日 · In this paper, we first screened 23 different syngeneic tumor models treated with anti-PD-1 and found that two colorectal carcinoma CT-26 and Colon 26 tumor models shared similar genetic background but exhibited different sensitivity to anti-PD-1 therapies.

2024年12月26日 · We established a cachexia model in BALB/c mice by transplanting C26 cells and administering 5-Aza on day 6 post-transplantation (Figure 3A). Treatment with 5-Aza significantly exacerbated weight loss and worsened overall health in C26 tumor-bearing mice compared with controls (Figures 3B and 3C).

In a syngeneic CT26 model, cancer cells from a particular mouse strain are implanted back into mice of the same strain, allowing researchers to study the interaction between the tumor and the immune system in a genetically matched system. The CT26 cell line is used to create the CT26 syngeneic murine model.

2021年6月26日 · In our study, the transcriptome of the cachectic gastrocnemius muscle in the C26 xenograft model was integrated and compared with that of 5 more different datasets. The bioinformatic analysis...

动物模型是深入研究cc的重要前提。目前荷c26 小鼠被认为是研究cc最为理想的动物模型，但荷 瘤小鼠进入恶病质不同阶段的判定标准仍有争议， 且在荷瘤小鼠恶病质整个自然发展过程方面缺乏 系统性研究。为此，本研究综合评估了荷c26小

2010年7月8日 · Mice bearing ectopically-implanted C26 colon carcinoma are widely used as an experimental model of cancer cachexia. As part of the search for novel clinical and basic research applications for this experimental model, we characterized novel cellular and molecular features of C26-bearing mice.

2019年7月24日 · Cancer cachexia was induced in male BALB/c mice by inoculation of mouse C26 colon adenocarcinoma cells, whereas spleen deficiency syndrome was induced by treating the mice with spleen...

2016年11月30日 · Mice bearing the Colon-26 (C26) carcinoma represent a classical model of cancer cachexia. Progressive muscle wasting occurs in association with tumor growth, over-expression of muscle-specific ubiquitin ligases, and reductions in muscle cross-sectional area.

2021年11月13日 · Results: We performed in vivo screening for anti-PD-1 therapy across 23 syngeneic tumor models and found that CT-26 and Colon 26, which are murine colorectal carcinoma derived from BALB/c mice, showed different sensitivity to anti-PD-1.

我们在 23 个同基因肿瘤模型中进行了抗 PD-1 治疗的体内筛选，发现 CT-26 和结肠 26 是源自 BALB/c 小鼠的小鼠结肠直肠癌，对抗 PD-1 显示出不同的敏感性。 CT-26 肿瘤小鼠对抗 PD-1 抗体的敏感性高于结肠 26，而两种模型对抗 CTLA4 抗体的敏感性相似。 免疫分析显示 CT-26 肿瘤组织比结肠 26 浸润了更多的免疫细胞。 基因组/转录组分析强调 Wnt 通路是 CT-26 和结肠 26 之间的潜在差异之一，表明 Wnt 活性在结肠 26 中高于结肠 26 CT-26。 CT-26 和结肠 26 同基 …

2019年2月21日 · The C26 model was established by subcutaneous injection of 2 × 10 5 C26 cells into the right hind leg. Two different animal experiments were conducted: Firstly, a cachexia group (n = 10) and a normal group (n = 5) were monitored at 23 days for refractory cachexia experiment.

In this paper, we first screened 23 different syngeneic tumor models treated with anti-PD-1 and found that two colorectal carcinoma CT-26 and Colon 26 tumor models shared similar genetic background but exhibited different sensitivity to anti-PD-1 therapies.

CT26 is an N-nitroso-N-methylurethane- (NNMU) induced, undifferentiated colon carcinoma cell line. It was cloned to generate the cell line designated CT26.WT (ATCC CRL-2638). Use CT26.WT mouse fibroblasts with CT26.CL25 (ATCC CRL-2639) as a model for testing immunotherapy protocols and in studies on the host immune response.

2017年3月1日 · We assessed thrombin generation and coagulation parameters in cachectic C26 tumor‐bearing mice. C26 mice are hypercoagulable, partially corrected by blocking tumor derived interleukin‐6. Coagulability and anti‐inflammatory interventions may be clinically important in cancer cachexia.

The present study aimed to determine the impact of colon 26 adenocarcinoma (C26)-induced cancer cachexia on skeletal muscle mitochondrial respiration and content. Twelve male CD2F1 mice were injected with C26-cells (tumor bearing (TB) group), whereas 12 age-matched mice received PBS vehicle injectio …

2022年3月5日 · The biodistribution and antitumor efficacy of commercially available doxorubicin (DXR)-containing PEGylated liposomes, Doxil®, were scrutinized in the anti-PEG IgM mouse model. In C26 tumor-bearing mice having circulating anti-PEG IgM, at 24 h after injection almost no DXR was observed in blood and tumor, and increased DXR accumulation was ...

CT-26 is a well established model for the study of physiopathology of colon cancers in humans. We have profiled the tumorigenicity of CT-26 mouse colon carcinoma as a flank model in BALB/c mice. Our model displays aggressive metastatic tumor kinetics as a syngeneic model for tailored immunotherapeutic drug discovery.