We have studied the secretion of proteins of the alternative pathway of complement C3, factor B and factor H by human umbilical vein endothelial cells (HUVEC). Results showed that factor H and factor B are quantitatively secreted in abundance whereas C3 could only be detected when the cells are main …

Here we characterize FH activity at the host cell surface in the presence of membrane-bound complement regulators, using human umbilical vein endothelial cells (HUVEC) as a model for self cells. We show that FH attached to these cells exerts complement regulatory activity in concert with the integral membrane regulators CD46, CD55 and CD59.

1999年2月1日 · As the receptor for C3b is known to be present on injured bovine endothelial cells, we investigated whether hypoxia or inflammatory mediators induce complement receptor type 1 (CR1; CD35) expression on HUVECs. CR1 protein expression increased 3.7 ± 0.6-fold as measured by ELISA on HUVECs following hypoxia (48 h, 1% O 2 ).

Factor H is a central regulator in plasma which protects host tissue from complement mediated damage. Here we characterize the relevance of surface attached factor H, and study the regulatory...

1997年7月1日 · We investigated whether exposure of human umbilical vein endothelial cells (HUVECs) to hypoxia and/or reoxygenation activates complement and decreases HUVEC-surface expression of the C3 regulatory proteins CD46 and CD55.

2003年5月1日 · HUVEC took up more serum-opsonized than -unopsonized zymosan as demonstrated by flow cytometry with fluorescence-labeled particles. The former uptake was inhibited in the presence of anti-C3c antibodies and thus complement-mediated. It probably occurred via CR1 (CD35), although participation of other receptors cannot be ruled out.

2015年6月8日 · Apoptotic HUVEC-C3 appeared blue under FRET imaging. 2:1 ratio of HepG2-DsRed:HUVEC-C3 co-culture showed the best tubular formation after two days, with HUVEC-C3 appearing to have the highest...

2021年4月14日 · Multiple endothelial cell types can be used: we usually use Human umbilical vein endothelial cells (HUVEC—a model of macrovascular EC) or Human dermal EC line (HMEC—a microvascular EC model) . HUVEC and HMEC-1 are grown in 24 well plates (approximately 4.5 × 10 6 per wells) at 37 °C until they reach 80% of confluence.

1997年7月1日 · 我们调查了人类脐静脉内皮细胞（HUVEC）暴露于缺氧和/或复氧是否激活补体并降低C3调节蛋白CD46和CD55的HUVEC表面表达。 方法和结果将HUVEC置于0、12或24小时缺氧（O2 = 1％），然后在30％人血清存在的情况下再充氧3小时（O2 = 21％）。 通过ELISA和流式细胞术评估CD46和CD55的C3沉积和HUVEC表面表达。 经过缺氧12或24小时，再充氧3小时的HUVEC上的C3沉积明显大于常氧HUVEC。 在复氧过程中抑制经典而非替代补体途径会减 …

2022年4月11日 · C3c (a common epitope to C3, C3(H2O), C3b, and iC3b) (which reflects C3 convertase activity and the early phase of the complement cascade) can be detected by polyclonal anti-C3c antibody. Antibody targeting C5b-9 reveals the final step of the cascade.