Plasmid pT-CA-GFP-IRES-mLumin from Dr. Jeanne Hardy's lab contains the inserts CA-GFP and mLumin and is published in J Biol Chem. 2011 Jul 15;286(28):24977-86. Epub 2011 May 10. This plasmid is available through Addgene.

2013年4月2日 · We have developed a robust reporter of caspase proteolytic activity, called caspase-activatable green fluorescent protein (CA-GFP). The caspases play central roles in homeostatic regulation, as they execute programmed cell death, and in drug design, as caspases are involved in diseases ranging from cancer to neurodegeneration.

1997年8月28日 · To combine the brightness of fluorescent indicators with the targetability of a biosynthetic indicator, we have used GFP, a spontaneously fluorescent protein from the jellyfish Aequorea victoria.

钙离子成像技术是利用可以感应Ca2+浓度的钙离子指示剂，检测的是细胞或组织中Ca2+的浓度变化，将钙浓度变化转化为荧光信号，从而使细胞电活动转化为可记录的光信号。 钙离子指示剂分为两种，一种为化学指示剂，另一种为现在普遍使用的遗传编码指示剂，例如GCaMP。 GCaMP由绿色荧光蛋白（green fluorescent protein , GFP）、钙调蛋白（calmodulin, CaM）和肌球蛋白轻链激酶的一段肽段序列M13构成。 当有Ca2+结合CaM时，CaM发生构象变化，其轻链区可以结 …

2020年2月1日 · The paper describes GFP phase separation-based kinase reporters SPARK. When kinase is inactive, SPARK is as bright as EGFP with homogenous distribution in the cell. When kinase is activated, SPARK rapidly (within 20-s) forms intensely bright puncta with 10-fold fluorescence increase at single pixel level.

2016年7月21日 · Here, we report the design and characterization of a GFP-based fluorogenic protease reporter, dubbed ZipGFP. ZipGFP-based TEV protease reporter increased fluorescence 10-fold after activation by protease. A ZipGFP-based executioner caspase reporter visualized apoptosis in live zebrafish embryos with spatiotemporal resolution.

GCaMP钙指示剂是指环形排列荧光蛋白cpGFP（circularly permuted GFP）的N末端和C末端分别连接M13和CaM构成的基于荧光强度的钙指示剂。 CaM与钙离子结合后发生变构，并以其铰链区和M13结合，三者相互作用引起GFP生色基团周围环境变化，进而引起GCaMP的荧光强度显著增强。

CA-GFP Is an Apoptosis Reporter in Mammalian Cells — While CA-GFP responds robustly to the activity of human caspase expressed heterologously in bacteria, perhaps the most significant ...

Plasmid CA-RIT-NFAT1 (IRES-GFP) from Dr. Anjana Rao's lab contains the insert CA-RIT-NFAT1 and is published in Immunity. 2015 Feb 6. pii: S1074-7613 (15)00032-1. doi: 10.1016/j.immuni.2015.01.006. This plasmid is available through Addgene.

Plasmid pCS2 CA-LRP6-GFP from Dr. Edward De Robertis's lab contains the insert LDL-receptor related protein 6 and is published in Cell. 2010 Dec 23. 143(7):1136-48. This plasmid is available through Addgene.