
pT-CA-GFP-IRES-mLumin - Addgene
Plasmid pT-CA-GFP-IRES-mLumin from Dr. Jeanne Hardy's lab contains the inserts CA-GFP and mLumin and is published in J Biol Chem. 2011 Jul 15;286(28):24977-86. Epub 2011 May 10. …
A Tunable, Modular Approach to Fluorescent Protease-Activated …
Apr 2, 2013 · We have developed a robust reporter of caspase proteolytic activity, called caspase-activatable green fluorescent protein (CA-GFP). The caspases play central roles in …
Fluorescent indicators for Ca 2+ based on green fluorescent
Aug 28, 1997 · To combine the brightness of fluorescent indicators with the targetability of a biosynthetic indicator, we have used GFP, a spontaneously fluorescent protein from the …
新一代无损伤钙离子探针“GCaMP-X” - 知乎专栏
钙离子成像技术是利用可以感应Ca2+浓度的钙离子指示剂,检测的是细胞或组织中Ca2+的浓度变化,将钙浓度变化转化为荧光信号,从而使细胞电活动转化为可记录的光信号。 钙离子指示 …
Imaging dynamic cell signaling - ScienceDirect
Feb 1, 2020 · The paper describes GFP phase separation-based kinase reporters SPARK. When kinase is inactive, SPARK is as bright as EGFP with homogenous distribution in the cell. When …
Rational Design of a GFP-Based Fluorogenic Caspase ... - Cell Press
Jul 21, 2016 · Here, we report the design and characterization of a GFP-based fluorogenic protease reporter, dubbed ZipGFP. ZipGFP-based TEV protease reporter increased …
荧光蛋白的升级用法 - 知乎 - 知乎专栏
GCaMP钙指示剂是指环形排列荧光蛋白cpGFP(circularly permuted GFP)的N末端和C末端分别连接M13和CaM构成的基于荧光强度的钙指示剂。 CaM与钙离子结合后发生变构,并以其铰 …
CA-GFP becomes fluorescent only upon cleavage by active …
CA-GFP Is an Apoptosis Reporter in Mammalian Cells — While CA-GFP responds robustly to the activity of human caspase expressed heterologously in bacteria, perhaps the most significant ...
CA-RIT-NFAT1 (IRES-GFP) - Addgene
Plasmid CA-RIT-NFAT1 (IRES-GFP) from Dr. Anjana Rao's lab contains the insert CA-RIT-NFAT1 and is published in Immunity. 2015 Feb 6. pii: S1074-7613 (15)00032-1. doi: …
pCS2 CA-LRP6-GFP - Addgene
Plasmid pCS2 CA-LRP6-GFP from Dr. Edward De Robertis's lab contains the insert LDL-receptor related protein 6 and is published in Cell. 2010 Dec 23. 143(7):1136-48. This plasmid is …
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