2015年8月4日 · Expression of YFP-Tagged βCA4 at the Plasma Membrane Rescues the Insensitive Stomatal Responses of ca1ca4 to CO 2 Changes. In contrast to CA1-YFP, YFP-tagged βCA4 was detected at the plasma membrane of ca1ca4 guard cells (Fig. 4A), consistent with transient expression in tobacco cells (Fabre et al., 2007; Hu et al., 2010).

2019年11月12日 · We previously showed that nearly 100% of hippocampal CA1 SST interneurons are labeled with YFP in Lhx6-Cre;R26R-YFP transgenic mice (Fogarty et al., 2007). We extended this work by assessing SST interneurons at all anterior-posterior levels of CA1. We confirmed a near-complete co-localization of SST with YFP (Figures 3 A–3C).

Our results identify mechanisms and genes that function in the repression of stomatal development in leaves during atmospheric CO 2 elevation, including the carbonic-anhydrase-encoding genes CA1 and CA4 and the secreted protease CRSP, which cleaves the pro-peptide EPF2, in turn repressing stomatal development.

2015年8月4日 · Microscopic analysis of YFP-tagged βCA1 showed that the N terminus spanning the first 65 amino acids of βCA1 containing the chloroplast peptide signal was targeted to chloroplasts, whereas expression of a truncated βCA1 without the first 65 amino acids (CA1 66–330) was localized in the vicinity of the plasma membrane or cytoplasm .

2021年7月22日 · Here, we describe the coarse topography of the chicken ASIC1 intracellular domains determined by fluorescence resonance energy transfer (FRET), measured using either fluorescent lifetime imaging or patch clamp fluorometry.

2024年1月22日 · In addition, YFP expression was occasionally seen in putative CA1 pyramidal neurons. While this off-target effect does lessen the specificity of mscRE4 in granule cell studies, it could provide an avenue for experiments requiring highly sparse expression in CA1 in mice and potentially across other species.

In these rats, Halorhodopsin (NpHR-YFP, n = 4 rats) or Archaerhodopsin (ArchT, n = 1 rat) was expressed in the dorsal CA1 region of the hippocampus under the control of the CaMKIIα promoter using an adeno-associated virus (AAV2/1).

2018年2月19日 · Here we show that single presynaptic axons form multiple, spatially clustered inputs onto the distal, but not proximal, dendrites of CA1 pyramidal neurons. These compound connections exhibit...

2020年10月30日 · To examine the function of the MS-CA1 pathway in memory retrieval, we first injected into the dorsal CA1 region a herpes simplex virus expressing yellow fluorescent protein (HSV-YFP) for high-efficiency brain-wide retrograde cell tagging (fig. S1, A to C).

2014年7月6日 · Our results identify mechanisms and genes that function in the repression of stomatal development in leaves during atmospheric CO 2 elevation, including the carbonic-anhydrase-encoding genes CA1...