The CCA sequence is conserved at the 3' end of all mature tRNA molecules to function as the site of amino acid attachment. This sequence is acquired and maintained by stepwise nucleotide addition by the ubiquitous CCA enzyme, which is an unusual RNA ...

2018年4月1日 · Recent discoveries have revealed that the CCA-adding enzyme – actually responsible for the de novo synthesis of the 3′-CCA end – plays an indispensable role in tRNA quality control by incorporating a second CCA triplet that is recognized as a degradation tag. In this review, we give an update on the latest findings regarding tRNA quality ...

The CCA sequence is common to all the 3' ends of tRNAs and more than half of the tRNAs possess the G1C72 base pair at the end of acceptor stem. To study the role of these terminal trinucleotides and their vicinity in the aminoacylation process, not only the substitutions of these bases but also the …

We show that the ancestral candidate catalyzes an error-free CCA-addition, but has a much higher tRNA affinity compared to the extant enzyme. The consequence of this increased substrate binding is an enhanced reverse reaction, where the enzyme removes the …

Recent discoveries have revealed that the CCA-adding enzyme - actually responsible for the de novo synthesis of the 3'-CCA end - plays an indispensable role in tRNA quality control by incorporating a second CCA triplet that is recognized as a degradation tag.

2004年8月5日 · Transfer RNA nucleotidyltransferases (CCA-adding enzymes) are responsible for the maturation or repair of the functional 3′ end of tRNAs by means of the...

2016年12月8日 · By templating CCA in tRNA fMet genes, Bacteria can directly synthesize tRNA fMet with the CCA sequence at the 3′-end. Below we hypothesize five non-mutually exclusive potential advantages for tRNA genes to template CCA.

1979年1月1日 · The chapter summarizes recent results on the function of the -N-C-C-A end of tRNA as a reactive site during aminoacylation and ribosomal protein biosynthesis, with information about the structure of -N-C-C-A end and changes in its function after its modification.

Cytoplasmic tRNAs undergo posttranscriptional 5' and 3' end processing in the eukaryotic nucleus, and CCA (which forms the mature 3' end of all tRNAs) must be added by tRNA nucleotidyl transferase before tRNA can be aminoacylated and utilized in ...

tRNA nucleotidyltransferase adds the invariant CCA-terminus to the tRNA 3'-end, a central step in tRNA maturation. This CCA-adding enzyme is a specialized RNA polymerase that synthesizes the CCA sequence at high fidelity in all kingdoms of life.